Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18678 | 3' | -56.4 | NC_004682.1 | + | 1246 | 0.67 | 0.548984 |
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Target: 5'- ---gGCggCGGUCACCcucggaGACGaggaGGCUGCu -3' miRNA: 3'- gaagCGa-GCCAGUGG------CUGCg---UCGACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 33892 | 0.67 | 0.570491 |
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Target: 5'- cCUUCGCuUCGGcCccuGCCGACGacgaGGCccagGCg -3' miRNA: 3'- -GAAGCG-AGCCaG---UGGCUGCg---UCGa---CG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 35197 | 0.67 | 0.592197 |
|
Target: 5'- -aUCGCgcagugcgUGGUgGCCGGCGguG-UGCg -3' miRNA: 3'- gaAGCGa-------GCCAgUGGCUGCguCgACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 45647 | 0.66 | 0.624983 |
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Target: 5'- --aUGCUCGccgCGCUGGgGCuGCUGCu -3' miRNA: 3'- gaaGCGAGCca-GUGGCUgCGuCGACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 4764 | 0.66 | 0.635937 |
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Target: 5'- --cUGCUCGaUCagGCCGACGUAGUcggUGCc -3' miRNA: 3'- gaaGCGAGCcAG--UGGCUGCGUCG---ACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 46337 | 0.76 | 0.164449 |
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Target: 5'- gUUCGCUCGGgCGCUGGCG-AGCUGa -3' miRNA: 3'- gAAGCGAGCCaGUGGCUGCgUCGACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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