Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18678 | 3' | -56.4 | NC_004682.1 | + | 3117 | 0.68 | 0.527736 |
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Target: 5'- -aUCGC-CGGuuacgggucgaUCACUGGCGCAGgCgGCg -3' miRNA: 3'- gaAGCGaGCC-----------AGUGGCUGCGUC-GaCG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 27547 | 0.68 | 0.538324 |
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Target: 5'- -gUCGCUaCGGcggcgaCACUGGCGCuGCgGCa -3' miRNA: 3'- gaAGCGA-GCCa-----GUGGCUGCGuCGaCG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 5059 | 0.68 | 0.538324 |
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Target: 5'- ---gGCUaCGcGUaCGCCGACGCcgcaccGGCUGCu -3' miRNA: 3'- gaagCGA-GC-CA-GUGGCUGCG------UCGACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 8978 | 0.67 | 0.548984 |
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Target: 5'- gCUUCgGCUCGG-CggggccgauaagGCCGACgaGCAGUUGUg -3' miRNA: 3'- -GAAG-CGAGCCaG------------UGGCUG--CGUCGACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 27424 | 0.67 | 0.559709 |
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Target: 5'- -aUCGCcgUCGGcgCACUGGCGUucucGCUGUc -3' miRNA: 3'- gaAGCG--AGCCa-GUGGCUGCGu---CGACG- -5' |
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| 18678 | 3' | -56.4 | NC_004682.1 | + | 9434 | 1.1 | 0.000573 |
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Target: 5'- gCUUCGCUCGGUCACCGACGCAGCUGCc -3' miRNA: 3'- -GAAGCGAGCCAGUGGCUGCGUCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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