Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18678 | 5' | -56.8 | NC_004682.1 | + | 26378 | 0.66 | 0.551975 |
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Target: 5'- aAUGCUcGggGUcGGCcACUGCGGGGCc -3' miRNA: 3'- -UACGA-CuaCGuCCGcUGACGUCUCGa -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 20136 | 0.66 | 0.530028 |
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Target: 5'- -gGCUGAUGCGGGCuGA--GCAGcGUUg -3' miRNA: 3'- uaCGACUACGUCCG-CUgaCGUCuCGA- -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 3138 | 0.66 | 0.508407 |
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Target: 5'- --aCUGgcGCAGGCGGC-GCAGcAGCc -3' miRNA: 3'- uacGACuaCGUCCGCUGaCGUC-UCGa -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 34761 | 0.66 | 0.493497 |
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Target: 5'- --cCUGAUGCGGGCGACguucucgccugugGCAGAc-- -3' miRNA: 3'- uacGACUACGUCCGCUGa------------CGUCUcga -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 5198 | 0.67 | 0.456126 |
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Target: 5'- -gGCUGAaGCAGGcCGACUGCAc---- -3' miRNA: 3'- uaCGACUaCGUCC-GCUGACGUcucga -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 11260 | 0.67 | 0.446016 |
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Target: 5'- uUGC-GGUaGCGGGCGGCUucugaGCGGAGUc -3' miRNA: 3'- uACGaCUA-CGUCCGCUGA-----CGUCUCGa -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 13774 | 0.68 | 0.42618 |
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Target: 5'- -aGUcGAUGCGGGCGG-UGCgccgAGAGCUg -3' miRNA: 3'- uaCGaCUACGUCCGCUgACG----UCUCGA- -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 34396 | 0.69 | 0.342974 |
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Target: 5'- -cGCgGAUGCuGGcCGACUacgggcgGCAGGGCUa -3' miRNA: 3'- uaCGaCUACGuCC-GCUGA-------CGUCUCGA- -5' |
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| 18678 | 5' | -56.8 | NC_004682.1 | + | 9470 | 1.06 | 0.000667 |
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Target: 5'- gAUGCUGAUGCAGGCGACUGCAGAGCUg -3' miRNA: 3'- -UACGACUACGUCCGCUGACGUCUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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