Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18680 | 5' | -61.1 | NC_004682.1 | + | 18763 | 0.7 | 0.231597 |
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Target: 5'- cGGUCGUCUGGUUCUucuccaucUGGAGAcGCUGAc -3' miRNA: 3'- uCCGGUAGGCCAGGA--------GCCUCU-CGGCU- -5' |
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| 18680 | 5' | -61.1 | NC_004682.1 | + | 40885 | 0.71 | 0.193612 |
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Target: 5'- uGGUCGUCCacGGUggCgCUCGGGGGGCUGAc -3' miRNA: 3'- uCCGGUAGG--CCA--G-GAGCCUCUCGGCU- -5' |
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| 18680 | 5' | -61.1 | NC_004682.1 | + | 2076 | 0.71 | 0.17907 |
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Target: 5'- -cGCCGU-CGG-CCUCGGGGGGCCa- -3' miRNA: 3'- ucCGGUAgGCCaGGAGCCUCUCGGcu -5' |
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| 18680 | 5' | -61.1 | NC_004682.1 | + | 13512 | 0.74 | 0.123358 |
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Target: 5'- aAGGCCAgUCCGGUaCgUCGGcacgacGGGCCGAg -3' miRNA: 3'- -UCCGGU-AGGCCA-GgAGCCu-----CUCGGCU- -5' |
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| 18680 | 5' | -61.1 | NC_004682.1 | + | 428 | 0.74 | 0.116852 |
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Target: 5'- gGGaGCCGcuugggggCGGcUCCUCGGAGGGCCGAg -3' miRNA: 3'- -UC-CGGUag------GCC-AGGAGCCUCUCGGCU- -5' |
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| 18680 | 5' | -61.1 | NC_004682.1 | + | 9759 | 1.08 | 0.000313 |
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Target: 5'- gAGGCCAUCCGGUCCUCGGAGAGCCGAc -3' miRNA: 3'- -UCCGGUAGGCCAGGAGCCUCUCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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