Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18683 | 3' | -55.1 | NC_004682.1 | + | 25250 | 0.66 | 0.668872 |
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Target: 5'- gGCAaGGuAGCCCaC-CGCGUCAAGg-- -3' miRNA: 3'- -UGUaCC-UCGGGcGuGCGCAGUUCaag -5' |
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| 18683 | 3' | -55.1 | NC_004682.1 | + | 49843 | 0.68 | 0.579647 |
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Target: 5'- cACGUGGucCCCGCACGaaugCGGGUUg -3' miRNA: 3'- -UGUACCucGGGCGUGCgca-GUUCAAg -5' |
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| 18683 | 3' | -55.1 | NC_004682.1 | + | 17317 | 0.68 | 0.568597 |
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Target: 5'- gGCGUcGGAGCCCgaGCugGCcaggGUCAGGa-- -3' miRNA: 3'- -UGUA-CCUCGGG--CGugCG----CAGUUCaag -5' |
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| 18683 | 3' | -55.1 | NC_004682.1 | + | 1537 | 0.68 | 0.5576 |
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Target: 5'- uGCcgGGaAGUugacgggcucgCCGCugGCGUCGAGaUUCu -3' miRNA: 3'- -UGuaCC-UCG-----------GGCGugCGCAGUUC-AAG- -5' |
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| 18683 | 3' | -55.1 | NC_004682.1 | + | 47718 | 0.7 | 0.432365 |
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Target: 5'- cCGUGuGGCCCGCcuucuugguCGCGUgCAGGUUCu -3' miRNA: 3'- uGUACcUCGGGCGu--------GCGCA-GUUCAAG- -5' |
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| 18683 | 3' | -55.1 | NC_004682.1 | + | 10465 | 1.09 | 0.000847 |
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Target: 5'- aACAUGGAGCCCGCACGCGUCAAGUUCu -3' miRNA: 3'- -UGUACCUCGGGCGUGCGCAGUUCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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