Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18686 | 3' | -57.7 | NC_004682.1 | + | 33557 | 0.66 | 0.520109 |
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Target: 5'- cAGGCCGCUgGCgGCgGCAACGGc-- -3' miRNA: 3'- cUUCGGCGGgCGaUGgCGUUGCUcuu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 33480 | 0.66 | 0.529686 |
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Target: 5'- gGAGGCC-CCCaacggggagaagcGCUACUGCGcccacgGCGAGAu -3' miRNA: 3'- -CUUCGGcGGG-------------CGAUGGCGU------UGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 45654 | 0.66 | 0.530754 |
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Target: 5'- --cGCCGCCaUGCUcGCCGCGcUGGGGc -3' miRNA: 3'- cuuCGGCGG-GCGA-UGGCGUuGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 33888 | 0.66 | 0.530754 |
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Target: 5'- --cGCUucgGCCC-CUGCCGaCGACGAGGc -3' miRNA: 3'- cuuCGG---CGGGcGAUGGC-GUUGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 17800 | 0.66 | 0.541477 |
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Target: 5'- -cGGCC-CUCGCcACCGCGAUGGGu- -3' miRNA: 3'- cuUCGGcGGGCGaUGGCGUUGCUCuu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 16737 | 0.66 | 0.541477 |
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Target: 5'- --uGCCGCCCGagcgcaUGCCGCGcaGCGccugcuGGAAc -3' miRNA: 3'- cuuCGGCGGGCg-----AUGGCGU--UGC------UCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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