Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18686 | 3' | -57.7 | NC_004682.1 | + | 39821 | 0.66 | 0.515875 |
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Target: 5'- --cGCCGCCCgaugccucggcugcGCUcuugccaGCCGCgAACGGGAc -3' miRNA: 3'- cuuCGGCGGG--------------CGA-------UGGCG-UUGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 42394 | 0.68 | 0.373422 |
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Target: 5'- cGGAGCUGgCU-CUACCGCAcCGAGGAa -3' miRNA: 3'- -CUUCGGCgGGcGAUGGCGUuGCUCUU- -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 43987 | 0.68 | 0.391332 |
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Target: 5'- -cAGCCGCCUgGCUcCCGCAG-GGGAGc -3' miRNA: 3'- cuUCGGCGGG-CGAuGGCGUUgCUCUU- -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 44742 | 0.67 | 0.428787 |
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Target: 5'- ---aCCGCaaGCU-CCGCGACGAGGGu -3' miRNA: 3'- cuucGGCGggCGAuGGCGUUGCUCUU- -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 45654 | 0.66 | 0.530754 |
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Target: 5'- --cGCCGCCaUGCUcGCCGCGcUGGGGc -3' miRNA: 3'- cuuCGGCGG-GCGA-UGGCGUuGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 46153 | 0.66 | 0.520109 |
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Target: 5'- cGAAGCCGacgaCUGCggagaccagcacUGCCGCugcgacGACGAGGAa -3' miRNA: 3'- -CUUCGGCg---GGCG------------AUGGCG------UUGCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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