Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18686 | 3' | -57.7 | NC_004682.1 | + | 12885 | 0.72 | 0.238116 |
Target: 5'- uGAGCCGCUCGCUgGCgGCGuCGGGGGg -3' miRNA: 3'- cUUCGGCGGGCGA-UGgCGUuGCUCUU- -5' |
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18686 | 3' | -57.7 | NC_004682.1 | + | 11190 | 1.06 | 0.000702 |
Target: 5'- aGAAGCCGCCCGCUACCGCAACGAGAAg -3' miRNA: 3'- -CUUCGGCGGGCGAUGGCGUUGCUCUU- -5' |
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18686 | 3' | -57.7 | NC_004682.1 | + | 7433 | 0.69 | 0.356076 |
Target: 5'- cGggGCCGCagaGUU-CCGCgAGCGAGAGc -3' miRNA: 3'- -CuuCGGCGgg-CGAuGGCG-UUGCUCUU- -5' |
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18686 | 3' | -57.7 | NC_004682.1 | + | 6430 | 0.69 | 0.331136 |
Target: 5'- cGAGCacCGCCCGC-GCCGUgucGGCGGGGAg -3' miRNA: 3'- cUUCG--GCGGGCGaUGGCG---UUGCUCUU- -5' |
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18686 | 3' | -57.7 | NC_004682.1 | + | 4621 | 0.71 | 0.24442 |
Target: 5'- gGAGGCCacGCUCGCUGCCGagauCGAGGc -3' miRNA: 3'- -CUUCGG--CGGGCGAUGGCguu-GCUCUu -5' |
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18686 | 3' | -57.7 | NC_004682.1 | + | 2862 | 0.68 | 0.409793 |
Target: 5'- gGGAGuuGCCCaggaagauCCGCGACGGGAu -3' miRNA: 3'- -CUUCggCGGGcgau----GGCGUUGCUCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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