Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18686 | 3' | -57.7 | NC_004682.1 | + | 28696 | 0.7 | 0.307506 |
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Target: 5'- cGAuGCCGCCCacucGCgGCgGCGGCGGGAc -3' miRNA: 3'- -CUuCGGCGGG----CGaUGgCGUUGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 21818 | 0.69 | 0.339304 |
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Target: 5'- --uGCCGauCCCGCUGCUGgagacCAGCGAGAc -3' miRNA: 3'- cuuCGGC--GGGCGAUGGC-----GUUGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 39821 | 0.66 | 0.515875 |
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Target: 5'- --cGCCGCCCgaugccucggcugcGCUcuugccaGCCGCgAACGGGAc -3' miRNA: 3'- cuuCGGCGGG--------------CGA-------UGGCG-UUGCUCUu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 16210 | 0.66 | 0.520109 |
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Target: 5'- aAGGCCGCaCuCGCUcaguucGCCGCGAUGAu-- -3' miRNA: 3'- cUUCGGCG-G-GCGA------UGGCGUUGCUcuu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 17800 | 0.66 | 0.541477 |
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Target: 5'- -cGGCC-CUCGCcACCGCGAUGGGu- -3' miRNA: 3'- cuUCGGcGGGCGaUGGCGUUGCUCuu -5' |
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| 18686 | 3' | -57.7 | NC_004682.1 | + | 11190 | 1.06 | 0.000702 |
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Target: 5'- aGAAGCCGCCCGCUACCGCAACGAGAAg -3' miRNA: 3'- -CUUCGGCGGGCGAUGGCGUUGCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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