Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18692 | 3' | -56.9 | NC_004682.1 | + | 40231 | 0.68 | 0.472712 |
Target: 5'- -gGCCGACGAacACGGCcuggucAAUGCCCAGu -3' miRNA: 3'- agUGGCUGCUccUGCCG------UUGUGGGUC- -5' |
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18692 | 3' | -56.9 | NC_004682.1 | + | 42535 | 0.68 | 0.472712 |
Target: 5'- aC-CCGACGuGGcuCGGCGGCGCCUc- -3' miRNA: 3'- aGuGGCUGCuCCu-GCCGUUGUGGGuc -5' |
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18692 | 3' | -56.9 | NC_004682.1 | + | 43604 | 0.66 | 0.624693 |
Target: 5'- aCAgCGACGgcaacgugaucguccGGGAcuacaagacCGGCAACACCCc- -3' miRNA: 3'- aGUgGCUGC---------------UCCU---------GCCGUUGUGGGuc -5' |
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18692 | 3' | -56.9 | NC_004682.1 | + | 44796 | 0.67 | 0.555559 |
Target: 5'- cCACCGGCGGcGuCGGCA-UGCCCGa -3' miRNA: 3'- aGUGGCUGCUcCuGCCGUuGUGGGUc -5' |
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18692 | 3' | -56.9 | NC_004682.1 | + | 46137 | 0.68 | 0.513453 |
Target: 5'- gCAgCGcCGAGcGACaGCAGCACCgAGg -3' miRNA: 3'- aGUgGCuGCUC-CUGcCGUUGUGGgUC- -5' |
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18692 | 3' | -56.9 | NC_004682.1 | + | 46571 | 0.66 | 0.62034 |
Target: 5'- cCGCCG-CGccuGGAUcgcaGGCuuCGCCCAGa -3' miRNA: 3'- aGUGGCuGCu--CCUG----CCGuuGUGGGUC- -5' |
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18692 | 3' | -56.9 | NC_004682.1 | + | 47251 | 0.71 | 0.344883 |
Target: 5'- cCGCCGACGAGG-CGGCAccgacggaggccACAUCgGa -3' miRNA: 3'- aGUGGCUGCUCCuGCCGU------------UGUGGgUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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