Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18692 | 3' | -56.9 | NC_004682.1 | + | 44796 | 0.67 | 0.555559 |
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Target: 5'- cCACCGGCGGcGuCGGCA-UGCCCGa -3' miRNA: 3'- aGUGGCUGCUcCuGCCGUuGUGGGUc -5' |
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| 18692 | 3' | -56.9 | NC_004682.1 | + | 35464 | 0.67 | 0.544926 |
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Target: 5'- gUCAUCGaggacucccGCGAGGGCugGGCGGCugCCc- -3' miRNA: 3'- -AGUGGC---------UGCUCCUG--CCGUUGugGGuc -5' |
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| 18692 | 3' | -56.9 | NC_004682.1 | + | 32245 | 0.67 | 0.538578 |
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Target: 5'- gUCAcCCGGCGAGGucaacaccaugcGCcugaugaagcgagcuGGCuuCACCCAGg -3' miRNA: 3'- -AGU-GGCUGCUCC------------UG---------------CCGuuGUGGGUC- -5' |
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| 18692 | 3' | -56.9 | NC_004682.1 | + | 13270 | 0.67 | 0.53436 |
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Target: 5'- aUCACCGucauGCGAGaucuGCGGUA--GCCCAGg -3' miRNA: 3'- -AGUGGC----UGCUCc---UGCCGUugUGGGUC- -5' |
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| 18692 | 3' | -56.9 | NC_004682.1 | + | 36404 | 0.68 | 0.523866 |
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Target: 5'- gUCACCcGCGAcGACGGCccuGGCACUCGc -3' miRNA: 3'- -AGUGGcUGCUcCUGCCG---UUGUGGGUc -5' |
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| 18692 | 3' | -56.9 | NC_004682.1 | + | 35036 | 0.68 | 0.513453 |
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Target: 5'- -gGCCGugGAGGAaGGCuggGCugGCCCGa -3' miRNA: 3'- agUGGCugCUCCUgCCGu--UG--UGGGUc -5' |
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| 18692 | 3' | -56.9 | NC_004682.1 | + | 21257 | 0.66 | 0.642107 |
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Target: 5'- uUCGCCGugGAGGuaGCGcCGACACaCGa -3' miRNA: 3'- -AGUGGCugCUCC--UGCcGUUGUGgGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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