miRNA: 3'- -GCGac-GACUUGGUGUGcAUGCGGaug -5'
miRNA: 3'- -GCGACGacu-UGGugUGC---AUGCGGaUG- -5'
miRNA: 3'- gCGACgaCUUGGUguGCaUGCGGAUG- -5'
miRNA: 3'- -GCGa---CGACUuggUGUGCAUGCGGAuG- -5'
miRNA: 3'- -GCgACGACU---UGGUGUGC---AUGCGGa--UG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18698 | 5' | -53.7 | NC_004682.1 | + | 10584 | 0.66 | 0.73984 |
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Target: 5'- gCGCgacCUGGGCCACGCcUGCGUCa-- -3' miRNA: 3'- -GCGac-GACUUGGUGUGcAUGCGGaug -5' |
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| 18698 | 5' | -53.7 | NC_004682.1 | + | 10009 | 0.66 | 0.761031 |
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Target: 5'- uCGCUGCgucuGCCugGCGgcgUACGUCgGCg -3' miRNA: 3'- -GCGACGacu-UGGugUGC---AUGCGGaUG- -5' |
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| 18698 | 5' | -53.7 | NC_004682.1 | + | 8950 | 0.66 | 0.761031 |
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Target: 5'- uGCUGggGGACCG--UG-ACGCCUACg -3' miRNA: 3'- gCGACgaCUUGGUguGCaUGCGGAUG- -5' |
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| 18698 | 5' | -53.7 | NC_004682.1 | + | 6753 | 0.66 | 0.771425 |
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Target: 5'- aCGCgcacGCUGGug-ACAUGUACGCCUcCa -3' miRNA: 3'- -GCGa---CGACUuggUGUGCAUGCGGAuG- -5' |
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| 18698 | 5' | -53.7 | NC_004682.1 | + | 6113 | 0.66 | 0.771425 |
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Target: 5'- aCGaUGCUGGcccGCUACGCGgccUACGCCaagGCg -3' miRNA: 3'- -GCgACGACU---UGGUGUGC---AUGCGGa--UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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