Results 61 - 68 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18700 | 3' | -53.9 | NC_004682.1 | + | 6072 | 0.67 | 0.683475 |
Target: 5'- aCGACGCCaauACCGGCGgGCUggugagCCGUuGGc -3' miRNA: 3'- aGUUGCGG---UGGUUGC-CGAa-----GGCGuUC- -5' |
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18700 | 3' | -53.9 | NC_004682.1 | + | 5116 | 0.69 | 0.560719 |
Target: 5'- gUCGACGCCgcGCCGAUccuGGCgcgugCUGCGGGc -3' miRNA: 3'- -AGUUGCGG--UGGUUG---CCGaa---GGCGUUC- -5' |
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18700 | 3' | -53.9 | NC_004682.1 | + | 5072 | 0.72 | 0.405907 |
Target: 5'- cCGACGCCGCac-CGGCUgcucCCGCAc- -3' miRNA: 3'- aGUUGCGGUGguuGCCGAa---GGCGUuc -5' |
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18700 | 3' | -53.9 | NC_004682.1 | + | 4979 | 0.73 | 0.378245 |
Target: 5'- gUCuggauCGCCA-CAGCGGCUUccagCCGCAGGg -3' miRNA: 3'- -AGuu---GCGGUgGUUGCCGAA----GGCGUUC- -5' |
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18700 | 3' | -53.9 | NC_004682.1 | + | 3827 | 0.67 | 0.676806 |
Target: 5'- cCAACGCCgaguacugcugguauGCCAaaGCGGCguagUCGCGAc -3' miRNA: 3'- aGUUGCGG---------------UGGU--UGCCGaa--GGCGUUc -5' |
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18700 | 3' | -53.9 | NC_004682.1 | + | 1452 | 0.68 | 0.627575 |
Target: 5'- cUCGACGCCAgCGGCGaGCccgucaacUUCCcgGCAGGc -3' miRNA: 3'- -AGUUGCGGUgGUUGC-CG--------AAGG--CGUUC- -5' |
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18700 | 3' | -53.9 | NC_004682.1 | + | 553 | 0.71 | 0.485489 |
Target: 5'- -gAGCGCCGCCGgaaGCGGCccuggaggCCGCuGGa -3' miRNA: 3'- agUUGCGGUGGU---UGCCGaa------GGCGuUC- -5' |
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18700 | 3' | -53.9 | NC_004682.1 | + | 352 | 0.66 | 0.727342 |
Target: 5'- cCGAggaGCCGCCcccaAGCGGCUccccUCCGgGAGc -3' miRNA: 3'- aGUUg--CGGUGG----UUGCCGA----AGGCgUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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