Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18700 | 3' | -53.9 | NC_004682.1 | + | 16324 | 0.66 | 0.748733 |
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Target: 5'- aCAcCGCgACCAGCGGUcgCuCGCAu- -3' miRNA: 3'- aGUuGCGgUGGUUGCCGaaG-GCGUuc -5' |
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| 18700 | 3' | -53.9 | NC_004682.1 | + | 33795 | 0.66 | 0.748733 |
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Target: 5'- -gAAgGCCccgGCCAagGCGGCUcCCGcCAAGg -3' miRNA: 3'- agUUgCGG---UGGU--UGCCGAaGGC-GUUC- -5' |
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| 18700 | 3' | -53.9 | NC_004682.1 | + | 27465 | 0.66 | 0.723014 |
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Target: 5'- cCGugGCCACCcguacCGGCgauagaauccucaUCUGCAAGu -3' miRNA: 3'- aGUugCGGUGGuu---GCCGa------------AGGCGUUC- -5' |
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| 18700 | 3' | -53.9 | NC_004682.1 | + | 41611 | 0.67 | 0.705558 |
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Target: 5'- aCAACGUgACCAACG---UCCGCAc- -3' miRNA: 3'- aGUUGCGgUGGUUGCcgaAGGCGUuc -5' |
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| 18700 | 3' | -53.9 | NC_004682.1 | + | 33871 | 0.67 | 0.694548 |
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Target: 5'- aCGACGagGCCcaGGCGGCUcCCGcCGAGg -3' miRNA: 3'- aGUUGCggUGG--UUGCCGAaGGC-GUUC- -5' |
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| 18700 | 3' | -53.9 | NC_004682.1 | + | 49661 | 0.67 | 0.694548 |
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Target: 5'- uUCAACuggGCCgagacGCUGACGGUggUUCGCAAGg -3' miRNA: 3'- -AGUUG---CGG-----UGGUUGCCGa-AGGCGUUC- -5' |
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| 18700 | 3' | -53.9 | NC_004682.1 | + | 3827 | 0.67 | 0.676806 |
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Target: 5'- cCAACGCCgaguacugcugguauGCCAaaGCGGCguagUCGCGAc -3' miRNA: 3'- aGUUGCGG---------------UGGU--UGCCGaa--GGCGUUc -5' |
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| 18700 | 3' | -53.9 | NC_004682.1 | + | 11867 | 0.66 | 0.779865 |
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Target: 5'- aCGACGCCGa-GACGGCaccggUCCaGUggGg -3' miRNA: 3'- aGUUGCGGUggUUGCCGa----AGG-CGuuC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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