Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18700 | 5' | -63.5 | NC_004682.1 | + | 3228 | 0.73 | 0.091125 |
Target: 5'- gGCgGCGCGGCAcaggGCGacaUC-GGCGGCg -3' miRNA: 3'- gCGaCGCGCCGUa---CGCg--AGcCCGCCG- -5' |
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18700 | 5' | -63.5 | NC_004682.1 | + | 2963 | 0.66 | 0.306435 |
Target: 5'- gCGC-GgGCGGCG-GCGCU-GGGCaGUg -3' miRNA: 3'- -GCGaCgCGCCGUaCGCGAgCCCGcCG- -5' |
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18700 | 5' | -63.5 | NC_004682.1 | + | 2081 | 0.68 | 0.217518 |
Target: 5'- gGgUGCGCcGUcgGC-CUCGGGgGGCc -3' miRNA: 3'- gCgACGCGcCGuaCGcGAGCCCgCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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