Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18701 | 3' | -56.7 | NC_004682.1 | + | 30637 | 0.66 | 0.57253 |
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Target: 5'- -uCGUCCgGuCGCCCGacagcGCGGCCAg -3' miRNA: 3'- uuGUAGGaCuGCGGGCau---CGCUGGUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 1639 | 0.66 | 0.538627 |
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Target: 5'- aGGCAUCUggUGGCGaCCCGUuggcgugGGgGACCGUc -3' miRNA: 3'- -UUGUAGG--ACUGC-GGGCA-------UCgCUGGUA- -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 47193 | 0.67 | 0.507551 |
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Target: 5'- cGCAUCCUcGACGCCUG-AGCcAUCAc -3' miRNA: 3'- uUGUAGGA-CUGCGGGCaUCGcUGGUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 4457 | 0.68 | 0.426298 |
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Target: 5'- cGCGcUCC--GCGCCCGUGGCGGCgGc -3' miRNA: 3'- uUGU-AGGacUGCGGGCAUCGCUGgUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 35676 | 0.69 | 0.388669 |
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Target: 5'- cACG-CCUGAUGCCCacagGUGGCGuCCAc -3' miRNA: 3'- uUGUaGGACUGCGGG----CAUCGCuGGUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 9594 | 0.71 | 0.312352 |
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Target: 5'- cGCAUCCUGGCGUUCG-AG-GACCAc -3' miRNA: 3'- uUGUAGGACUGCGGGCaUCgCUGGUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 34316 | 0.73 | 0.211414 |
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Target: 5'- ----cCCUGcCGCCCGUAGuCGGCCAg -3' miRNA: 3'- uuguaGGACuGCGGGCAUC-GCUGGUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 30720 | 0.74 | 0.184593 |
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Target: 5'- cGCGUCCUGGcCGCgCUGUcgGGCGACCGg -3' miRNA: 3'- uUGUAGGACU-GCG-GGCA--UCGCUGGUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 24707 | 0.79 | 0.083534 |
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Target: 5'- cGACAUCCUGGCaccggaGCCCGUGGCGuucGCCGg -3' miRNA: 3'- -UUGUAGGACUG------CGGGCAUCGC---UGGUa -5' |
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| 18701 | 3' | -56.7 | NC_004682.1 | + | 17657 | 1.04 | 0.001244 |
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Target: 5'- cAACAUCCUGACGCCCGUAGCGACCAUg -3' miRNA: 3'- -UUGUAGGACUGCGGGCAUCGCUGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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