Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18701 | 5' | -59.4 | NC_004682.1 | + | 30491 | 0.67 | 0.428959 |
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Target: 5'- uCGACgGCUucGACCGggccuacccCGGCG-UGCAGCGGc -3' miRNA: 3'- -GCUG-CGA--CUGGU---------GCCGCgACGUCGUC- -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 30815 | 0.74 | 0.134753 |
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Target: 5'- aGACGCUGcCCAgCGGCgacugGCUGguGCGGc -3' miRNA: 3'- gCUGCGACuGGU-GCCG-----CGACguCGUC- -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 33556 | 0.71 | 0.24158 |
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Target: 5'- aGGcCGCUGGCgGCGGCaacgGCGGCAGc -3' miRNA: 3'- gCU-GCGACUGgUGCCGcga-CGUCGUC- -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 37615 | 0.67 | 0.392305 |
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Target: 5'- cCGACGCcGGCC-CGGUGgaGCAaggGCAc -3' miRNA: 3'- -GCUGCGaCUGGuGCCGCgaCGU---CGUc -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 38101 | 0.71 | 0.234872 |
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Target: 5'- uCGGCGCUGAggcaccucggcaCCGaggaGGCGCUGUcgaagaucuacgaGGCAGg -3' miRNA: 3'- -GCUGCGACU------------GGUg---CCGCGACG-------------UCGUC- -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 39316 | 0.79 | 0.059288 |
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Target: 5'- uCGACGCUGAgCCGCuuccgcuugccguuGGCGCgGCGGCAGc -3' miRNA: 3'- -GCUGCGACU-GGUG--------------CCGCGaCGUCGUC- -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 42537 | 0.68 | 0.333268 |
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Target: 5'- cCGACG-UGGCUcgGCGGCGCcucUGCGGCc- -3' miRNA: 3'- -GCUGCgACUGG--UGCCGCG---ACGUCGuc -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 43093 | 0.72 | 0.206869 |
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Target: 5'- cCGACGCUGugCgcguACGGCcUUGCGGUGGu -3' miRNA: 3'- -GCUGCGACugG----UGCCGcGACGUCGUC- -5' |
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| 18701 | 5' | -59.4 | NC_004682.1 | + | 48252 | 0.7 | 0.247817 |
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Target: 5'- aGAUGCUcGCC-CGGUGUgGCGGCAGc -3' miRNA: 3'- gCUGCGAcUGGuGCCGCGaCGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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