Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18704 | 3' | -57.1 | NC_004682.1 | + | 40338 | 0.66 | 0.604959 |
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Target: 5'- uGGUUCaCC-GCGUcgaGCGcGGUC-ACCGCGa -3' miRNA: 3'- -CCAAG-GGuUGCA---CGC-CCAGcUGGCGU- -5' |
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| 18704 | 3' | -57.1 | NC_004682.1 | + | 45613 | 0.67 | 0.581294 |
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Target: 5'- ---cCCCGGCGcagGCGGGUCcaacuugugaacACCGCAg -3' miRNA: 3'- ccaaGGGUUGCa--CGCCCAGc-----------UGGCGU- -5' |
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| 18704 | 3' | -57.1 | NC_004682.1 | + | 20278 | 0.67 | 0.562075 |
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Target: 5'- cGUgaagCCCuccguCGUGC-GGUCGACCcGCAc -3' miRNA: 3'- cCAa---GGGuu---GCACGcCCAGCUGG-CGU- -5' |
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| 18704 | 3' | -57.1 | NC_004682.1 | + | 20342 | 0.67 | 0.552529 |
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Target: 5'- cGUUCuCCGACGagaccugcuccacacGCGGGUCGguaucgacaACCGCGu -3' miRNA: 3'- cCAAG-GGUUGCa--------------CGCCCAGC---------UGGCGU- -5' |
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| 18704 | 3' | -57.1 | NC_004682.1 | + | 46425 | 0.68 | 0.520056 |
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Target: 5'- cGUUCCCucgcaccCG-GCGGGcUCGggGCCGCAc -3' miRNA: 3'- cCAAGGGuu-----GCaCGCCC-AGC--UGGCGU- -5' |
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| 18704 | 3' | -57.1 | NC_004682.1 | + | 6405 | 0.68 | 0.499507 |
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Target: 5'- gGGgagUCCCGGacccaGCGGGUCGGCCu-- -3' miRNA: 3'- -CCa--AGGGUUgca--CGCCCAGCUGGcgu -5' |
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| 18704 | 3' | -57.1 | NC_004682.1 | + | 27763 | 0.69 | 0.42136 |
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Target: 5'- aGGagCCaCAGCG-GCGGGaUCG-CCGCGa -3' miRNA: 3'- -CCaaGG-GUUGCaCGCCC-AGCuGGCGU- -5' |
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| 18704 | 3' | -57.1 | NC_004682.1 | + | 20185 | 1.1 | 0.000562 |
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Target: 5'- cGGUUCCCAACGUGCGGGUCGACCGCAc -3' miRNA: 3'- -CCAAGGGUUGCACGCCCAGCUGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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