Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 18704 | 5' | -58.9 | NC_004682.1 | + | 23901 | 0.69 | 0.304227 |
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Target: 5'- -gCAcCGGCCACaCCcccGAGGACGCGGc -3' miRNA: 3'- aaGU-GCCGGUGcGGu--CUCUUGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 98 | 0.69 | 0.327365 |
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Target: 5'- cUCGCGGgcgaCugGaCAGAGAACGgGGUa -3' miRNA: 3'- aAGUGCCg---GugCgGUCUCUUGCgCCA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 21521 | 0.7 | 0.26177 |
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Target: 5'- -cCACGGCguguaguacuCGCCGGGGAACGuCGGg -3' miRNA: 3'- aaGUGCCGgu--------GCGGUCUCUUGC-GCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 37980 | 0.71 | 0.212813 |
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Target: 5'- -cUACGGCCAccCGCgAGAGAAcuuCGCGGa -3' miRNA: 3'- aaGUGCCGGU--GCGgUCUCUU---GCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 42719 | 0.74 | 0.154241 |
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Target: 5'- gUCGCGGCCuccaGCGUCGG-GAACGgGGUu -3' miRNA: 3'- aAGUGCCGG----UGCGGUCuCUUGCgCCA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 1480 | 0.75 | 0.130785 |
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Target: 5'- gUCGCGaCCGCGCCAGAGGACcaGCGaGUc -3' miRNA: 3'- aAGUGCcGGUGCGGUCUCUUG--CGC-CA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 25342 | 0.79 | 0.059175 |
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Target: 5'- -aCugGGCCACGCCGuGGGccuuGACGCGGUg -3' miRNA: 3'- aaGugCCGGUGCGGU-CUC----UUGCGCCA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 20222 | 1.04 | 0.00082 |
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Target: 5'- cUUCACGGCCACGCCAGAGAACGCGGUu -3' miRNA: 3'- -AAGUGCCGGUGCGGUCUCUUGCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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