Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18704 | 5' | -58.9 | NC_004682.1 | + | 25342 | 0.79 | 0.059175 |
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Target: 5'- -aCugGGCCACGCCGuGGGccuuGACGCGGUg -3' miRNA: 3'- aaGugCCGGUGCGGU-CUC----UUGCGCCA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 35176 | 0.66 | 0.491855 |
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Target: 5'- --gGCGGUgUGCGCCGGucAGCGCGGa -3' miRNA: 3'- aagUGCCG-GUGCGGUCucUUGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 37322 | 0.67 | 0.383629 |
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Target: 5'- -cCACGGCCACcguGgCGGcauccggcugaaccAGAACGCGGg -3' miRNA: 3'- aaGUGCCGGUG---CgGUC--------------UCUUGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 37980 | 0.71 | 0.212813 |
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Target: 5'- -cUACGGCCAccCGCgAGAGAAcuuCGCGGa -3' miRNA: 3'- aaGUGCCGGU--GCGgUCUCUU---GCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 42719 | 0.74 | 0.154241 |
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Target: 5'- gUCGCGGCCuccaGCGUCGG-GAACGgGGUu -3' miRNA: 3'- aAGUGCCGG----UGCGGUCuCUUGCgCCA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 43414 | 0.66 | 0.481684 |
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Target: 5'- ---gUGGCCcCGCgAGAGGGuCGCGGa -3' miRNA: 3'- aaguGCCGGuGCGgUCUCUU-GCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 47286 | 0.67 | 0.432458 |
|
Target: 5'- aUCuccuCGGCCAgCGCCAG-GAcuaGCGGg -3' miRNA: 3'- aAGu---GCCGGU-GCGGUCuCUug-CGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 49573 | 0.67 | 0.385404 |
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Target: 5'- gUCACGGCUACGCCuacuGAGAggaaucaACGauGa -3' miRNA: 3'- aAGUGCCGGUGCGGu---CUCU-------UGCgcCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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