Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18704 | 5' | -58.9 | NC_004682.1 | + | 7855 | 0.67 | 0.432458 |
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Target: 5'- cUUCuCGGCCACggugucguuGCCGGGGAuguGCGCGu- -3' miRNA: 3'- -AAGuGCCGGUG---------CGGUCUCU---UGCGCca -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 6129 | 0.66 | 0.481684 |
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Target: 5'- -aCGCGGCCuACGCCAaGGcguacGACgGCGGg -3' miRNA: 3'- aaGUGCCGG-UGCGGUcUC-----UUG-CGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 5199 | 0.66 | 0.442071 |
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Target: 5'- -cCGCaGCaCGCGCCAG-GAucgGCGCGGc -3' miRNA: 3'- aaGUGcCG-GUGCGGUCuCU---UGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 4374 | 0.67 | 0.432458 |
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Target: 5'- gUCACcgaaGCCGcCGCCAcgggcgcGGAGCGCGGUg -3' miRNA: 3'- aAGUGc---CGGU-GCGGUc------UCUUGCGCCA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 3175 | 0.67 | 0.432458 |
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Target: 5'- -cCACGGCgG-GCCAGG--GCGCGGg -3' miRNA: 3'- aaGUGCCGgUgCGGUCUcuUGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 3076 | 0.67 | 0.386293 |
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Target: 5'- -cCACGggaugucgacGCCGCGUCGGAGggUGaCGGa -3' miRNA: 3'- aaGUGC----------CGGUGCGGUCUCuuGC-GCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 1480 | 0.75 | 0.130785 |
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Target: 5'- gUCGCGaCCGCGCCAGAGGACcaGCGaGUc -3' miRNA: 3'- aAGUGCcGGUGCGGUCUCUUG--CGC-CA- -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 98 | 0.69 | 0.327365 |
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Target: 5'- cUCGCGGgcgaCugGaCAGAGAACGgGGUa -3' miRNA: 3'- aAGUGCCg---GugCgGUCUCUUGCgCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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