Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18704 | 5' | -58.9 | NC_004682.1 | + | 16430 | 0.67 | 0.404368 |
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Target: 5'- --gACGGCCAgucgacCGUCGuuGAGGGCGCGGc -3' miRNA: 3'- aagUGCCGGU------GCGGU--CUCUUGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 18657 | 0.67 | 0.404368 |
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Target: 5'- gUUugGGCUcguaGCGCCAGAG-ACGCu-- -3' miRNA: 3'- aAGugCCGG----UGCGGUCUCuUGCGcca -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 7855 | 0.67 | 0.432458 |
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Target: 5'- cUUCuCGGCCACggugucguuGCCGGGGAuguGCGCGu- -3' miRNA: 3'- -AAGuGCCGGUG---------CGGUCUCU---UGCGCca -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 23986 | 0.66 | 0.442071 |
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Target: 5'- aUCGCGgcaGCCGCGuCCuGGGggUGUGGc -3' miRNA: 3'- aAGUGC---CGGUGC-GGuCUCuuGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 5199 | 0.66 | 0.442071 |
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Target: 5'- -cCGCaGCaCGCGCCAG-GAucgGCGCGGc -3' miRNA: 3'- aaGUGcCG-GUGCGGUCuCU---UGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 43414 | 0.66 | 0.481684 |
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Target: 5'- ---gUGGCCcCGCgAGAGGGuCGCGGa -3' miRNA: 3'- aaguGCCGGuGCGgUCUCUU-GCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 35176 | 0.66 | 0.491855 |
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Target: 5'- --gGCGGUgUGCGCCGGucAGCGCGGa -3' miRNA: 3'- aagUGCCG-GUGCGGUCucUUGCGCCa -5' |
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| 18704 | 5' | -58.9 | NC_004682.1 | + | 25342 | 0.79 | 0.059175 |
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Target: 5'- -aCugGGCCACGCCGuGGGccuuGACGCGGUg -3' miRNA: 3'- aaGugCCGGUGCGGU-CUC----UUGCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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