Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18705 | 3' | -59.8 | NC_004682.1 | + | 22023 | 0.66 | 0.470478 |
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Target: 5'- aUGGUGUGGgCCGUg--UGCGCCCuGGu -3' miRNA: 3'- -ACCACGUUgGGCAaggACGCGGG-CCu -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 47051 | 0.66 | 0.460673 |
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Target: 5'- gUGGUGUucuGACCCaugacucaCCUaGcCGCCCGGAu -3' miRNA: 3'- -ACCACG---UUGGGcaa-----GGA-C-GCGGGCCU- -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 48687 | 0.66 | 0.460673 |
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Target: 5'- aGGcGCAGuuCCCGaacgCCacgcgGUGCCCGGAg -3' miRNA: 3'- aCCaCGUU--GGGCaa--GGa----CGCGGGCCU- -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 23874 | 0.66 | 0.460673 |
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Target: 5'- cGGU-CAACaaGUUCCUGgGCCUGa- -3' miRNA: 3'- aCCAcGUUGggCAAGGACgCGGGCcu -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 30642 | 0.66 | 0.441395 |
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Target: 5'- cGGUcgcccgacagcGCGGCCaggacgcggaGUUCCUGgGCCUGGu -3' miRNA: 3'- aCCA-----------CGUUGGg---------CAAGGACgCGGGCCu -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 27804 | 0.66 | 0.431929 |
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Target: 5'- ---cGCAGCuuGa--CUGCGCCCGGu -3' miRNA: 3'- accaCGUUGggCaagGACGCGGGCCu -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 25732 | 0.71 | 0.226243 |
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Target: 5'- cGGuUGguACCCGgaCCccaguggaGCGCCCGGAc -3' miRNA: 3'- aCC-ACguUGGGCaaGGa-------CGCGGGCCU- -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 5475 | 0.73 | 0.165149 |
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Target: 5'- cGGcGCGGCCUGguacuggacggUCgaGCGCCCGGAc -3' miRNA: 3'- aCCaCGUUGGGCa----------AGgaCGCGGGCCU- -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 45530 | 0.73 | 0.160789 |
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Target: 5'- cGGUGUucacaaguugGACCCG--CCUGCGCCgGGGc -3' miRNA: 3'- aCCACG----------UUGGGCaaGGACGCGGgCCU- -5' |
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| 18705 | 3' | -59.8 | NC_004682.1 | + | 20866 | 1.08 | 0.000353 |
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Target: 5'- cUGGUGCAACCCGUUCCUGCGCCCGGAg -3' miRNA: 3'- -ACCACGUUGGGCAAGGACGCGGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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