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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18706 | 3' | -59.6 | NC_004682.1 | + | 2063 | 0.66 | 0.481442 |
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Target: 5'- cUGG-UCCgcaGGGuccGACCggCCCGCAGGg -3' miRNA: 3'- cACCaAGGa--CCC---CUGGagGGGUGUCC- -5' |
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| 18706 | 3' | -59.6 | NC_004682.1 | + | 429 | 0.66 | 0.481442 |
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Target: 5'- -gGGgagccgCUUGGGGGCggCUCCUCGgAGGg -3' miRNA: 3'- caCCaa----GGACCCCUG--GAGGGGUgUCC- -5' |
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| 18706 | 3' | -59.6 | NC_004682.1 | + | 25747 | 0.66 | 0.452118 |
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Target: 5'- -cGGcucgCCUucGGGGAUCUCCCCugGu- -3' miRNA: 3'- caCCaa--GGA--CCCCUGGAGGGGugUcc -5' |
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| 18706 | 3' | -59.6 | NC_004682.1 | + | 8367 | 0.76 | 0.113668 |
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Target: 5'- gGUGGcguccacgUCCUcgcgGGGGACCUCCCCGCucucguaguccucGGGg -3' miRNA: 3'- -CACCa-------AGGA----CCCCUGGAGGGGUG-------------UCC- -5' |
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| 18706 | 3' | -59.6 | NC_004682.1 | + | 21467 | 1.09 | 0.000355 |
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Target: 5'- cGUGGUUCCUGGGGACCUCCCCACAGGc -3' miRNA: 3'- -CACCAAGGACCCCUGGAGGGGUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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