Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18713 | 5' | -51.3 | NC_004682.1 | + | 859 | 0.67 | 0.864783 |
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Target: 5'- uUCGGUCGGucGCUUCcUCCugGCUCGu -3' miRNA: 3'- cAGCUAGUCucUGAAGuAGG--CGAGCu -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 31216 | 0.67 | 0.847565 |
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Target: 5'- --aGcgCAGGGGCUUC--CUGCUCGAc -3' miRNA: 3'- cagCuaGUCUCUGAAGuaGGCGAGCU- -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 23373 | 0.67 | 0.83859 |
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Target: 5'- -aCGAcgCGGAGccggucucCUUCgAUCCGCUCGAu -3' miRNA: 3'- caGCUa-GUCUCu-------GAAG-UAGGCGAGCU- -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 13054 | 0.68 | 0.810319 |
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Target: 5'- --aGGUCcGGGAUgcgccgcuugaUCAUCCGCUCGAc -3' miRNA: 3'- cagCUAGuCUCUGa----------AGUAGGCGAGCU- -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 20216 | 0.68 | 0.780257 |
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Target: 5'- uGUCGGUCAGcGACgaga-CCGCUUGGa -3' miRNA: 3'- -CAGCUAGUCuCUGaaguaGGCGAGCU- -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 37624 | 0.69 | 0.759377 |
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Target: 5'- -cCGAUCAGGGACUUgA-CCGCcCGc -3' miRNA: 3'- caGCUAGUCUCUGAAgUaGGCGaGCu -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 14474 | 0.71 | 0.648662 |
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Target: 5'- -gCGGgucCAGGGAC-UCAUCCaGCUCGGg -3' miRNA: 3'- caGCUa--GUCUCUGaAGUAGG-CGAGCU- -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 17068 | 0.72 | 0.557133 |
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Target: 5'- cUCGAagacccCGGAGACcgccgucUUCAUCUGCUCGAa -3' miRNA: 3'- cAGCUa-----GUCUCUG-------AAGUAGGCGAGCU- -5' |
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| 18713 | 5' | -51.3 | NC_004682.1 | + | 25961 | 1.09 | 0.002237 |
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Target: 5'- gGUCGAUCAGAGACUUCAUCCGCUCGAa -3' miRNA: 3'- -CAGCUAGUCUCUGAAGUAGGCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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