Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18716 | 5' | -53.3 | NC_004682.1 | + | 40960 | 0.66 | 0.759292 |
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Target: 5'- cGCGAcUGGgucGCCCGGAccacGAUCggaacgCGCUCAa -3' miRNA: 3'- -UGCU-ACU---CGGGCCU----CUAGaa----GUGAGU- -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 16691 | 0.67 | 0.716348 |
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Target: 5'- gUGAUGGGCCUGGAGggCUcgGgUCGa -3' miRNA: 3'- uGCUACUCGGGCCUCuaGAagUgAGU- -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 9413 | 0.68 | 0.683182 |
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Target: 5'- cACGAcgGAGaucacCCCGGAG--CUUCGCUCGg -3' miRNA: 3'- -UGCUa-CUC-----GGGCCUCuaGAAGUGAGU- -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 31757 | 0.68 | 0.660797 |
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Target: 5'- -aGAUGAGCCCGGAcGAag-UCGCg-- -3' miRNA: 3'- ugCUACUCGGGCCU-CUagaAGUGagu -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 17724 | 0.69 | 0.582131 |
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Target: 5'- cCGAUGAuccccgGCCUGGucgAGAaCUUCGCUCAg -3' miRNA: 3'- uGCUACU------CGGGCC---UCUaGAAGUGAGU- -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 7805 | 0.71 | 0.516232 |
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Target: 5'- gGCGAcGGGCaCCGGGGAUCUUgG-UCAg -3' miRNA: 3'- -UGCUaCUCG-GGCCUCUAGAAgUgAGU- -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 19440 | 0.72 | 0.453537 |
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Target: 5'- -gGGUGAaucCCCGGAGGUC-UCGCUCu -3' miRNA: 3'- ugCUACUc--GGGCCUCUAGaAGUGAGu -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 6326 | 0.76 | 0.272173 |
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Target: 5'- ----aGAGCCCGGAGGUCggcgUCACUg- -3' miRNA: 3'- ugcuaCUCGGGCCUCUAGa---AGUGAgu -5' |
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| 18716 | 5' | -53.3 | NC_004682.1 | + | 28002 | 1.09 | 0.00145 |
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Target: 5'- cACGAUGAGCCCGGAGAUCUUCACUCAg -3' miRNA: 3'- -UGCUACUCGGGCCUCUAGAAGUGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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