Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18720 | 5' | -60.4 | NC_004682.1 | + | 9378 | 0.67 | 0.419931 |
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Target: 5'- aCCCACuGGCCGUCCUCgcgguuccagaUGACCguguuguUCGGcaGGu -3' miRNA: 3'- -GGGUG-CUGGCAGGAG-----------GCUGG-------AGCC--CC- -5' |
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| 18720 | 5' | -60.4 | NC_004682.1 | + | 26097 | 0.67 | 0.466902 |
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Target: 5'- gCCUACGGCgggaGUCCgacgCCGGCCUCa--- -3' miRNA: 3'- -GGGUGCUGg---CAGGa---GGCUGGAGcccc -5' |
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| 18720 | 5' | -60.4 | NC_004682.1 | + | 13178 | 0.66 | 0.49479 |
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Target: 5'- -gUACGuguaguuGCCGUCgguCUCCGAcagguagcCCUCGGGGu -3' miRNA: 3'- ggGUGC-------UGGCAG---GAGGCU--------GGAGCCCC- -5' |
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| 18720 | 5' | -60.4 | NC_004682.1 | + | 7685 | 0.66 | 0.495766 |
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Target: 5'- aCguCGGgCGguuaCCUUCGACCgaugCGGGGg -3' miRNA: 3'- gGguGCUgGCa---GGAGGCUGGa---GCCCC- -5' |
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| 18720 | 5' | -60.4 | NC_004682.1 | + | 4856 | 0.66 | 0.505567 |
|
Target: 5'- uCCCACaGCCgGUCCuUCUGAgCCUgCGuGGGc -3' miRNA: 3'- -GGGUGcUGG-CAGG-AGGCU-GGA-GC-CCC- -5' |
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| 18720 | 5' | -60.4 | NC_004682.1 | + | 44245 | 0.66 | 0.52541 |
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Target: 5'- aCCCACGAucCCGUCgucguCUCCGACCccCGa-- -3' miRNA: 3'- -GGGUGCU--GGCAG-----GAGGCUGGa-GCccc -5' |
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| 18720 | 5' | -60.4 | NC_004682.1 | + | 2090 | 0.79 | 0.06703 |
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Target: 5'- aCCCugGACgGgugcgCCgUCGGCCUCGGGGg -3' miRNA: 3'- -GGGugCUGgCa----GGaGGCUGGAGCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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