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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18730 | 5' | -56.5 | NC_004682.1 | + | 46491 | 0.66 | 0.539886 |
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Target: 5'- -gGCGGUcaCCAGCGCCUUG-GUGAg-- -3' miRNA: 3'- aaCGCUA--GGUCGUGGAGCgCACUgaa -5' |
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| 18730 | 5' | -56.5 | NC_004682.1 | + | 46137 | 0.67 | 0.47591 |
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Target: 5'- -gGCGAUCCAGCACCugcUCGaucaGgccGACg- -3' miRNA: 3'- aaCGCUAGGUCGUGG---AGCg---Ca--CUGaa -5' |
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| 18730 | 5' | -56.5 | NC_004682.1 | + | 43413 | 0.68 | 0.455421 |
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Target: 5'- -gGUGAUCCAcGCgGCgUgGCGUGGCUUg -3' miRNA: 3'- aaCGCUAGGU-CG-UGgAgCGCACUGAA- -5' |
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| 18730 | 5' | -56.5 | NC_004682.1 | + | 25118 | 0.7 | 0.318915 |
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Target: 5'- gUUGUGGUCCagacgaucucagAGCACCUCG-GUGACc- -3' miRNA: 3'- -AACGCUAGG------------UCGUGGAGCgCACUGaa -5' |
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| 18730 | 5' | -56.5 | NC_004682.1 | + | 41172 | 0.73 | 0.221399 |
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Target: 5'- cUUGCGAUCCAGCGCgUCGag-GGCg- -3' miRNA: 3'- -AACGCUAGGUCGUGgAGCgcaCUGaa -5' |
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| 18730 | 5' | -56.5 | NC_004682.1 | + | 34648 | 1.02 | 0.00151 |
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Target: 5'- gUUGCGAUCCAGCACCUCGCGUGACUUc -3' miRNA: 3'- -AACGCUAGGUCGUGGAGCGCACUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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