Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18732 | 3' | -56.1 | NC_004682.1 | + | 522 | 0.68 | 0.555856 |
Target: 5'- aGGGAGGUGGUcuCGUU-GUCCuggucaACCAGu -3' miRNA: 3'- -CCCUCCACUAucGCGAgCAGG------UGGUC- -5' |
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18732 | 3' | -56.1 | NC_004682.1 | + | 15632 | 0.69 | 0.462365 |
Target: 5'- cGGGGGaagccgAGCGCUCGcCCGCCuGa -3' miRNA: 3'- cCCUCCacua--UCGCGAGCaGGUGGuC- -5' |
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18732 | 3' | -56.1 | NC_004682.1 | + | 28742 | 0.71 | 0.390269 |
Target: 5'- cGGGuaggugucgucguuGGUGGUGGUGCUCGUCUcCCc- -3' miRNA: 3'- -CCCu-------------CCACUAUCGCGAGCAGGuGGuc -5' |
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18732 | 3' | -56.1 | NC_004682.1 | + | 35757 | 1.1 | 0.000713 |
Target: 5'- gGGGAGGUGAUAGCGCUCGUCCACCAGc -3' miRNA: 3'- -CCCUCCACUAUCGCGAGCAGGUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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