Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18736 | 5' | -51.6 | NC_004682.1 | + | 39067 | 0.66 | 0.882671 |
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Target: 5'- ---uUCCGGGUGGUagCGAUGAAUCa-- -3' miRNA: 3'- caacAGGUCCAUCA--GCUACUUGGcga -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 15550 | 0.66 | 0.849364 |
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Target: 5'- --gGgagCCGGuUGGUCGAUGAAcuCCGCg -3' miRNA: 3'- caaCa--GGUCcAUCAGCUACUU--GGCGa -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 29663 | 0.67 | 0.821789 |
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Target: 5'- ----aCCAGGUGGUCGAggaucuccUGAGCCu-- -3' miRNA: 3'- caacaGGUCCAUCAGCU--------ACUUGGcga -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 39303 | 0.67 | 0.80232 |
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Target: 5'- ---uUCguGGUcguGGUCGAcgcUGAGCCGCUu -3' miRNA: 3'- caacAGguCCA---UCAGCU---ACUUGGCGA- -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 40722 | 0.68 | 0.786188 |
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Target: 5'- gGUUGUCCGGGUuaccuccaccggacgGGUCGGU--ACCGa- -3' miRNA: 3'- -CAACAGGUCCA---------------UCAGCUAcuUGGCga -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 35480 | 0.71 | 0.593241 |
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Target: 5'- --aGUCCGGGUGGUCc--GggUCGCa -3' miRNA: 3'- caaCAGGUCCAUCAGcuaCuuGGCGa -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 29320 | 0.71 | 0.57064 |
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Target: 5'- ---cUCCuGGUGGUCGGugccgacgUGGACCGCg -3' miRNA: 3'- caacAGGuCCAUCAGCU--------ACUUGGCGa -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 35530 | 0.72 | 0.559415 |
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Target: 5'- ----aCCGGGccgUAGUCGAUGAACCGg- -3' miRNA: 3'- caacaGGUCC---AUCAGCUACUUGGCga -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 26806 | 0.85 | 0.092025 |
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Target: 5'- --cGUUCGGGUAGUCGGUGAACCGg- -3' miRNA: 3'- caaCAGGUCCAUCAGCUACUUGGCga -5' |
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| 18736 | 5' | -51.6 | NC_004682.1 | + | 39194 | 1.08 | 0.002266 |
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Target: 5'- gGUUGUCCAGGUAGUCGAUGAACCGCUg -3' miRNA: 3'- -CAACAGGUCCAUCAGCUACUUGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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