Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18737 | 5' | -57.8 | NC_004682.1 | + | 36788 | 0.66 | 0.590375 |
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Target: 5'- aCCGUGCCCgGAGauucgagcaGCUUCAGGUaGAg-- -3' miRNA: 3'- -GGUACGGGgCUC---------UGGGGUCCA-CUacu -5' |
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| 18737 | 5' | -57.8 | NC_004682.1 | + | 37394 | 0.66 | 0.579629 |
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Target: 5'- aCCggGUgagguacuCCCGAGGCCgCAGGUcGUGGu -3' miRNA: 3'- -GGuaCG--------GGGCUCUGGgGUCCAcUACU- -5' |
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| 18737 | 5' | -57.8 | NC_004682.1 | + | 36838 | 0.66 | 0.568924 |
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Target: 5'- gUCA-GCCUCGAuGACCCCAGGc----- -3' miRNA: 3'- -GGUaCGGGGCU-CUGGGGUCCacuacu -5' |
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| 18737 | 5' | -57.8 | NC_004682.1 | + | 35633 | 0.66 | 0.55827 |
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Target: 5'- uCCAg--CCCGAGACCCagcaauugaaCAGGUGcUGGg -3' miRNA: 3'- -GGUacgGGGCUCUGGG----------GUCCACuACU- -5' |
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| 18737 | 5' | -57.8 | NC_004682.1 | + | 11635 | 0.66 | 0.537139 |
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Target: 5'- --cUGUCCCGAG-CaCCCAGGUcgcacugacuGGUGAc -3' miRNA: 3'- gguACGGGGCUCuG-GGGUCCA----------CUACU- -5' |
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| 18737 | 5' | -57.8 | NC_004682.1 | + | 46346 | 0.69 | 0.381786 |
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Target: 5'- ---gGCCCCGAGcCCgCCGGGUGcgaggGAa -3' miRNA: 3'- gguaCGGGGCUCuGG-GGUCCACua---CU- -5' |
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| 18737 | 5' | -57.8 | NC_004682.1 | + | 40160 | 0.7 | 0.356131 |
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Target: 5'- ---gGCCCCGAGguacacagGCCCuCGGGaGAUGAg -3' miRNA: 3'- gguaCGGGGCUC--------UGGG-GUCCaCUACU- -5' |
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| 18737 | 5' | -57.8 | NC_004682.1 | + | 39645 | 1.1 | 0.000448 |
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Target: 5'- cCCAUGCCCCGAGACCCCAGGUGAUGAu -3' miRNA: 3'- -GGUACGGGGCUCUGGGGUCCACUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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