Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18738 | 3' | -56.6 | NC_004682.1 | + | 28269 | 0.69 | 0.428308 |
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Target: 5'- aGCcu-CAUCGGCUUCGGGGcCGGCg- -3' miRNA: 3'- -CGucuGUAGCCGAGGCUCCaGCUGgu -5' |
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| 18738 | 3' | -56.6 | NC_004682.1 | + | 46087 | 0.7 | 0.418892 |
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Target: 5'- cGCAGuCGUCGGCUUCGGcguGGuacUCGAuCCAc -3' miRNA: 3'- -CGUCuGUAGCCGAGGCU---CC---AGCU-GGU- -5' |
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| 18738 | 3' | -56.6 | NC_004682.1 | + | 41084 | 0.7 | 0.38252 |
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Target: 5'- cCAGACgGUCGGCUUCGucgcgaucAGGUCG-CCGa -3' miRNA: 3'- cGUCUG-UAGCCGAGGC--------UCCAGCuGGU- -5' |
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| 18738 | 3' | -56.6 | NC_004682.1 | + | 12657 | 0.72 | 0.324124 |
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Target: 5'- cGUAGACGaacUCGGCUggaCCGAGGgCGuCCAc -3' miRNA: 3'- -CGUCUGU---AGCCGA---GGCUCCaGCuGGU- -5' |
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| 18738 | 3' | -56.6 | NC_004682.1 | + | 12958 | 0.72 | 0.301208 |
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Target: 5'- cGCGGACAUCGcggcgcGUUCuCGGGGuaccUCGGCCAg -3' miRNA: 3'- -CGUCUGUAGC------CGAG-GCUCC----AGCUGGU- -5' |
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| 18738 | 3' | -56.6 | NC_004682.1 | + | 40656 | 1.1 | 0.000629 |
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Target: 5'- gGCAGACAUCGGCUCCGAGGUCGACCAu -3' miRNA: 3'- -CGUCUGUAGCCGAGGCUCCAGCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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