Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18739 | 5' | -57.5 | NC_004682.1 | + | 41737 | 1.08 | 0.000648 |
Target: 5'- gCUCCAGCCGGUCAGGAUCGACAGCGAc -3' miRNA: 3'- -GAGGUCGGCCAGUCCUAGCUGUCGCU- -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 42466 | 0.68 | 0.452959 |
Target: 5'- -aCUGGCUGGcCGGGGUCGGCcucccuGCGGc -3' miRNA: 3'- gaGGUCGGCCaGUCCUAGCUGu-----CGCU- -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 42726 | 0.68 | 0.46278 |
Target: 5'- cCUCCAGCguCGGgaaCGGGGUucgCGACGGCu- -3' miRNA: 3'- -GAGGUCG--GCCa--GUCCUA---GCUGUCGcu -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 43277 | 0.71 | 0.313214 |
Target: 5'- gUCCGGCUcugGGUCcGGGucaaagcgcUCGGCGGCGAu -3' miRNA: 3'- gAGGUCGG---CCAGuCCU---------AGCUGUCGCU- -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 48069 | 0.72 | 0.279579 |
Target: 5'- uUCCAGUCGGcgaugucucgaugggUCAGGccggUGACGGCGAg -3' miRNA: 3'- gAGGUCGGCC---------------AGUCCua--GCUGUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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