Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18739 | 5' | -57.5 | NC_004682.1 | + | 41293 | 0.74 | 0.208662 |
Target: 5'- gUCUGGCCGGUCucGGGGUCGACgcccAGCu- -3' miRNA: 3'- gAGGUCGGCCAG--UCCUAGCUG----UCGcu -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 25516 | 0.74 | 0.203244 |
Target: 5'- -gCCAGaaGGUCAGGAggGACAGCa- -3' miRNA: 3'- gaGGUCggCCAGUCCUagCUGUCGcu -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 32938 | 0.79 | 0.086795 |
Target: 5'- gCUCCAGCuCGGUCAGGAcCGAcCGGCu- -3' miRNA: 3'- -GAGGUCG-GCCAGUCCUaGCU-GUCGcu -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 29026 | 0.8 | 0.077493 |
Target: 5'- uCUCCAGCag--CGGGAUCGGCAGCGGc -3' miRNA: 3'- -GAGGUCGgccaGUCCUAGCUGUCGCU- -5' |
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18739 | 5' | -57.5 | NC_004682.1 | + | 41737 | 1.08 | 0.000648 |
Target: 5'- gCUCCAGCCGGUCAGGAUCGACAGCGAc -3' miRNA: 3'- -GAGGUCGGCCAGUCCUAGCUGUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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