Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1875 | 5' | -52.1 | NC_001347.2 | + | 79723 | 0.66 | 0.996138 |
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Target: 5'- aGCAgCG-UCgGCGUUuucagcGUCGGCUCCGg -3' miRNA: 3'- -CGUaGCuAGgUGCAAc-----UAGCCGAGGU- -5' |
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| 1875 | 5' | -52.1 | NC_001347.2 | + | 153611 | 0.66 | 0.996138 |
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Target: 5'- aGCAUUuGUCCACGaUG-UCGGCgaugaccggCCAg -3' miRNA: 3'- -CGUAGcUAGGUGCaACuAGCCGa--------GGU- -5' |
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| 1875 | 5' | -52.1 | NC_001347.2 | + | 45067 | 0.66 | 0.995768 |
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Target: 5'- -aAUCGAgccugCCACGUUGucacgcaucucggCGGCUCgCAg -3' miRNA: 3'- cgUAGCUa----GGUGCAACua-----------GCCGAG-GU- -5' |
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| 1875 | 5' | -52.1 | NC_001347.2 | + | 82090 | 0.66 | 0.993989 |
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Target: 5'- cCGUCGucugaugcAUCCACGg----CGGCUCCGc -3' miRNA: 3'- cGUAGC--------UAGGUGCaacuaGCCGAGGU- -5' |
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| 1875 | 5' | -52.1 | NC_001347.2 | + | 154098 | 0.67 | 0.990967 |
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Target: 5'- cGCAcgaagCGAUCCACGUaGGccaCGuGCUCCu -3' miRNA: 3'- -CGUa----GCUAGGUGCAaCUa--GC-CGAGGu -5' |
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| 1875 | 5' | -52.1 | NC_001347.2 | + | 129772 | 0.67 | 0.990967 |
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Target: 5'- aCGUCGGUgCACGUaGAgcagCGGC-CCGc -3' miRNA: 3'- cGUAGCUAgGUGCAaCUa---GCCGaGGU- -5' |
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| 1875 | 5' | -52.1 | NC_001347.2 | + | 49235 | 0.69 | 0.977009 |
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Target: 5'- aGCGUCG-UCCGCGcgGG-CGGCagcaUCCAg -3' miRNA: 3'- -CGUAGCuAGGUGCaaCUaGCCG----AGGU- -5' |
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| 1875 | 5' | -52.1 | NC_001347.2 | + | 20689 | 0.69 | 0.96587 |
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Target: 5'- uCGUCGuuUCCAC-UUGcUCGGCUCCc -3' miRNA: 3'- cGUAGCu-AGGUGcAACuAGCCGAGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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