Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18752 | 3' | -54.2 | NC_004683.1 | + | 28713 | 0.66 | 0.768421 |
Target: 5'- cCGUgucGACGAGCaUGgcUGCGGCACUc- -3' miRNA: 3'- -GCGaa-CUGCUCGaAC--ACGUCGUGGag -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 42004 | 0.66 | 0.747478 |
Target: 5'- gGUggGACGcAGCUcguUGUGCAGCcgauGCCg- -3' miRNA: 3'- gCGaaCUGC-UCGA---ACACGUCG----UGGag -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 38872 | 0.66 | 0.747478 |
Target: 5'- gCGCUUaacgauggucagGACGGuCUcGcGCGGCACCUCg -3' miRNA: 3'- -GCGAA------------CUGCUcGAaCaCGUCGUGGAG- -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 43741 | 0.66 | 0.726048 |
Target: 5'- aGCUgcgGcACGAGCaccUGCAGCACUUg -3' miRNA: 3'- gCGAa--C-UGCUCGaacACGUCGUGGAg -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 7291 | 0.66 | 0.715181 |
Target: 5'- cCGgUUGGCGAGUcgugGUGC-GUGCCUUg -3' miRNA: 3'- -GCgAACUGCUCGaa--CACGuCGUGGAG- -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 34905 | 0.68 | 0.648604 |
Target: 5'- uGCggcACGGGCUUGUuuGCGGCGCUg- -3' miRNA: 3'- gCGaacUGCUCGAACA--CGUCGUGGag -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 13654 | 0.68 | 0.603749 |
Target: 5'- gCGgUUGGCGAGCUugaugccgaucUGUGCGgccugagauGCACCg- -3' miRNA: 3'- -GCgAACUGCUCGA-----------ACACGU---------CGUGGag -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 24248 | 0.68 | 0.603749 |
Target: 5'- gCGCggucaUGACGcGGcCUUGUGCAGgucggcgauCGCCUCg -3' miRNA: 3'- -GCGa----ACUGC-UC-GAACACGUC---------GUGGAG- -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 26271 | 0.69 | 0.581433 |
Target: 5'- gGCg-GGCGAGCgcguugGUGUGGCGCCg- -3' miRNA: 3'- gCGaaCUGCUCGaa----CACGUCGUGGag -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 2366 | 0.69 | 0.559292 |
Target: 5'- uGCUcauGCGGG---GUGCAGCGCCUCg -3' miRNA: 3'- gCGAac-UGCUCgaaCACGUCGUGGAG- -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 37832 | 0.71 | 0.453351 |
Target: 5'- gGC-UGAUGGGCUUGUgacGCGGcCGCCUg -3' miRNA: 3'- gCGaACUGCUCGAACA---CGUC-GUGGAg -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 56809 | 0.74 | 0.317634 |
Target: 5'- gGCUcGuCGGGCUUGaUGC-GCGCCUCg -3' miRNA: 3'- gCGAaCuGCUCGAAC-ACGuCGUGGAG- -5' |
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18752 | 3' | -54.2 | NC_004683.1 | + | 1205 | 1.11 | 0.000856 |
Target: 5'- gCGCUUGACGAGCUUGUGCAGCACCUCg -3' miRNA: 3'- -GCGAACUGCUCGAACACGUCGUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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