miRNA: 3'- -CACCGUa-----CGGCUGcu----CGGU--UAGUCGU- -5'
miRNA: 3'- caCCGUACGGCUGCu-----CGGuuA--GUCGU- -5'
miRNA: 3'- caCCGUa-------CGGCUGCUCGGUUAGUCGU- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18752 | 5' | -55.7 | NC_004683.1 | + | 34515 | 0.68 | 0.550207 |
|
Target: 5'- uGUGGCGaugagaGCCGACaacaguGCCAugAUCGGCAu -3' miRNA: 3'- -CACCGUa-----CGGCUGcu----CGGU--UAGUCGU- -5' |
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| 18752 | 5' | -55.7 | NC_004683.1 | + | 12249 | 0.68 | 0.539374 |
|
Target: 5'- -cGGCGUGCCGAUGcccggcGCUggUggCGGCAu -3' miRNA: 3'- caCCGUACGGCUGCu-----CGGuuA--GUCGU- -5' |
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| 18752 | 5' | -55.7 | NC_004683.1 | + | 41039 | 0.81 | 0.08171 |
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Target: 5'- -cGGCGccuaccucGCCGGCGAGCUGAUCAGCAg -3' miRNA: 3'- caCCGUa-------CGGCUGCUCGGUUAGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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