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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18753 | 5' | -57.7 | NC_004683.1 | + | 37908 | 0.68 | 0.437848 |
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Target: 5'- cGCUgGCaCUUCCAGGUcccAC-CCCAGGc- -3' miRNA: 3'- -CGAgUG-GGAGGUCCA---UGaGGGUCCac -5' |
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| 18753 | 5' | -57.7 | NC_004683.1 | + | 28424 | 0.68 | 0.486912 |
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Target: 5'- cGCUCACgCUCUGGGcaaGCUaCCCcgauGGUGg -3' miRNA: 3'- -CGAGUGgGAGGUCCa--UGA-GGGu---CCAC- -5' |
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| 18753 | 5' | -57.7 | NC_004683.1 | + | 48133 | 0.67 | 0.497039 |
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Target: 5'- cGCUCGCgCCacagcacacauUCCAGGUGCUCaCCAagcGGc- -3' miRNA: 3'- -CGAGUG-GG-----------AGGUCCAUGAG-GGU---CCac -5' |
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| 18753 | 5' | -57.7 | NC_004683.1 | + | 7163 | 0.67 | 0.507259 |
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Target: 5'- gGCgUCGCUgagaCGGGUGCgCCCGGGUGg -3' miRNA: 3'- -CG-AGUGGgag-GUCCAUGaGGGUCCAC- -5' |
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| 18753 | 5' | -57.7 | NC_004683.1 | + | 1402 | 1.11 | 0.000451 |
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Target: 5'- cGCUCACCCUCCAGGUACUCCCAGGUGa -3' miRNA: 3'- -CGAGUGGGAGGUCCAUGAGGGUCCAC- -5' |
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| 18753 | 5' | -57.7 | NC_004683.1 | + | 48957 | 0.69 | 0.418101 |
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Target: 5'- cCUCACcgggucaCCUCCGGGUACuggUCCCAuGUGc -3' miRNA: 3'- cGAGUG-------GGAGGUCCAUG---AGGGUcCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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