Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18755 | 3' | -59.8 | NC_004683.1 | + | 49097 | 0.66 | 0.505885 |
Target: 5'- gAGCGCUucccggUCGgGcUUGAGGGCGG-CGGg -3' miRNA: 3'- -UCGCGG------AGCgC-AGCUCUCGUCaGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 55771 | 0.66 | 0.505885 |
Target: 5'- cGaCGCCa-GCGUUGAGcAGCGGaCGGUc -3' miRNA: 3'- uC-GCGGagCGCAGCUC-UCGUCaGCCA- -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 16516 | 0.66 | 0.460398 |
Target: 5'- cAGCGCCugcggguugagcccgUCGCGcUCGAucGCGGUCaGGa -3' miRNA: 3'- -UCGCGG---------------AGCGC-AGCUcuCGUCAG-CCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 22727 | 0.67 | 0.446986 |
Target: 5'- cGGCGCacgCGCGUCGAuGGGCGGcCuGa -3' miRNA: 3'- -UCGCGga-GCGCAGCU-CUCGUCaGcCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 25876 | 0.67 | 0.446036 |
Target: 5'- uGCGgCUCgGCGUCGAGuucgccgGGCAGacgcuggccgccUCGGUg -3' miRNA: 3'- uCGCgGAG-CGCAGCUC-------UCGUC------------AGCCA- -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 51190 | 0.67 | 0.418999 |
Target: 5'- cGCGCCggcCGCGcccCGccAGUAGUCGGUg -3' miRNA: 3'- uCGCGGa--GCGCa--GCucUCGUCAGCCA- -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 42545 | 0.67 | 0.410812 |
Target: 5'- uGGCGCCgCGCGacgcacaccaugagCGAGgccgagcgcgccgcGGCGGUCGGg -3' miRNA: 3'- -UCGCGGaGCGCa-------------GCUC--------------UCGUCAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 7945 | 0.67 | 0.409909 |
Target: 5'- uGCGCCUUG-GUCG--GGguGUCGGg -3' miRNA: 3'- uCGCGGAGCgCAGCucUCguCAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 18276 | 0.67 | 0.400942 |
Target: 5'- cGUGCagacacagCGCGUCGuGcuGCAGUCGGg -3' miRNA: 3'- uCGCGga------GCGCAGCuCu-CGUCAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 44453 | 0.69 | 0.333877 |
Target: 5'- cGGCGaUCUCGcCGUCGAu-GUAGUCGGc -3' miRNA: 3'- -UCGC-GGAGC-GCAGCUcuCGUCAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 52155 | 0.7 | 0.296319 |
Target: 5'- cGGuCGCCUccCGCGUCGAcgaucagccaGGGCGcGUCGGg -3' miRNA: 3'- -UC-GCGGA--GCGCAGCU----------CUCGU-CAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 2535 | 0.7 | 0.275404 |
Target: 5'- cAGCGCUUgCGCGaCGGGAaGCcGUCGGa -3' miRNA: 3'- -UCGCGGA-GCGCaGCUCU-CGuCAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 51056 | 0.71 | 0.249376 |
Target: 5'- cGGCGCCUUgGCGgCGGGuGCGGcCGGa -3' miRNA: 3'- -UCGCGGAG-CGCaGCUCuCGUCaGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 51961 | 0.71 | 0.249376 |
Target: 5'- cGGCGCCacucacggaUCGCGcgguggugcagcUCGAGcGCGGUCGGc -3' miRNA: 3'- -UCGCGG---------AGCGC------------AGCUCuCGUCAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 45552 | 0.71 | 0.243195 |
Target: 5'- gGGCGCgUCGCGcgCGAGAagugcuGCGG-CGGUg -3' miRNA: 3'- -UCGCGgAGCGCa-GCUCU------CGUCaGCCA- -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 159 | 0.72 | 0.219736 |
Target: 5'- aAGCGCCUCgGCGUCGAcaccccAGCAGccgcCGGUu -3' miRNA: 3'- -UCGCGGAG-CGCAGCUc-----UCGUCa---GCCA- -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 40242 | 0.73 | 0.168542 |
Target: 5'- cGCGCCgagcugcaggcgCGCGUCGAuGAGUuggAGUCGGa -3' miRNA: 3'- uCGCGGa-----------GCGCAGCU-CUCG---UCAGCCa -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 40874 | 0.74 | 0.160698 |
Target: 5'- --gGCCUCGcCGUCGGcGAGCGcGUCGGUc -3' miRNA: 3'- ucgCGGAGC-GCAGCU-CUCGU-CAGCCA- -5' |
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18755 | 3' | -59.8 | NC_004683.1 | + | 2351 | 1.06 | 0.000612 |
Target: 5'- cAGCGCCUCGCGUCGAGAGCAGUCGGUc -3' miRNA: 3'- -UCGCGGAGCGCAGCUCUCGUCAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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