Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18755 | 5' | -55.2 | NC_004683.1 | + | 20391 | 0.66 | 0.74629 |
Target: 5'- aCAUGAACgCGCUgCCGcCGUCGccGCc -3' miRNA: 3'- aGUGCUUG-GCGAgGGCaGCAGUa-CGu -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 41161 | 0.66 | 0.74629 |
Target: 5'- gCGCgGGGCCGCUUacuaCGUgGUgAUGCGa -3' miRNA: 3'- aGUG-CUUGGCGAGg---GCAgCAgUACGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 10189 | 0.66 | 0.714771 |
Target: 5'- gCGCGGuagGCCGCgaCC-UCGUUAUGCAg -3' miRNA: 3'- aGUGCU---UGGCGagGGcAGCAGUACGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 44355 | 0.66 | 0.714771 |
Target: 5'- gCGCGGaucGCCGCgucgagCCgCGUCGUCuucgGUGCc -3' miRNA: 3'- aGUGCU---UGGCGa-----GG-GCAGCAG----UACGu -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 40302 | 0.67 | 0.704089 |
Target: 5'- gCGCGGGCCuGCUCggCGUCGUag-GCAg -3' miRNA: 3'- aGUGCUUGG-CGAGg-GCAGCAguaCGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 40425 | 0.67 | 0.686857 |
Target: 5'- cCAcCGAGCCGCagugugggcacccgCCCGUgG-CAUGCAc -3' miRNA: 3'- aGU-GCUUGGCGa-------------GGGCAgCaGUACGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 27677 | 0.67 | 0.682527 |
Target: 5'- uUCGgGGACCGCUucacgauguaCCCGgacaCGUCGgcggGCAa -3' miRNA: 3'- -AGUgCUUGGCGA----------GGGCa---GCAGUa---CGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 42749 | 0.67 | 0.671669 |
Target: 5'- gCGCGAagcuGCUGUUgCCGcCGUCGUGUc -3' miRNA: 3'- aGUGCU----UGGCGAgGGCaGCAGUACGu -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 6217 | 0.68 | 0.627973 |
Target: 5'- aCGCGAaccaGCCGCgcgaCCCGUUGggCcgGCGg -3' miRNA: 3'- aGUGCU----UGGCGa---GGGCAGCa-GuaCGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 46610 | 0.68 | 0.617034 |
Target: 5'- cCGCGAcggcaaggccGCCGCgCCCGUCGaUCA-GCc -3' miRNA: 3'- aGUGCU----------UGGCGaGGGCAGC-AGUaCGu -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 45612 | 0.68 | 0.606108 |
Target: 5'- gUCGCGGcgccGCUGCUgCCGUgGUUgguaGUGCAg -3' miRNA: 3'- -AGUGCU----UGGCGAgGGCAgCAG----UACGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 48303 | 0.69 | 0.58433 |
Target: 5'- gCGCGAuUUGUUCCCGcCG-CGUGCAu -3' miRNA: 3'- aGUGCUuGGCGAGGGCaGCaGUACGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 46107 | 0.69 | 0.56271 |
Target: 5'- gCGCgGGACCGCUCggCCGccgCGUCggGCAg -3' miRNA: 3'- aGUG-CUUGGCGAG--GGCa--GCAGuaCGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 51111 | 0.69 | 0.551979 |
Target: 5'- aUCGCGGcgAUCG-UCUCGUCGUCggGCAu -3' miRNA: 3'- -AGUGCU--UGGCgAGGGCAGCAGuaCGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 48700 | 0.71 | 0.468935 |
Target: 5'- gUCGCGGAguCCGCgggCCCaGUCGggGUGCAu -3' miRNA: 3'- -AGUGCUU--GGCGa--GGG-CAGCagUACGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 25749 | 0.71 | 0.458993 |
Target: 5'- uUCAuCGGGCCGCUgCCGUCGagCAcGCu -3' miRNA: 3'- -AGU-GCUUGGCGAgGGCAGCa-GUaCGu -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 49356 | 0.71 | 0.449163 |
Target: 5'- cCACGuguGCCGCga-CGUCGUCggGCAg -3' miRNA: 3'- aGUGCu--UGGCGaggGCAGCAGuaCGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 37496 | 0.72 | 0.375015 |
Target: 5'- gUACGGGCCGCagCCCG-CGUCAucucaucugaUGCAu -3' miRNA: 3'- aGUGCUUGGCGa-GGGCaGCAGU----------ACGU- -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 36888 | 0.73 | 0.341175 |
Target: 5'- cUACGAGCCggugacgcagauGCUCCCacUCGUCGUGCu -3' miRNA: 3'- aGUGCUUGG------------CGAGGGc-AGCAGUACGu -5' |
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18755 | 5' | -55.2 | NC_004683.1 | + | 47015 | 0.77 | 0.194975 |
Target: 5'- gCGCGuGCCGCUCCac-CGUCGUGCAc -3' miRNA: 3'- aGUGCuUGGCGAGGgcaGCAGUACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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