Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18756 | 3' | -60.9 | NC_004683.1 | + | 51965 | 0.68 | 0.309466 |
Target: 5'- gUUCCGgcgccacucacggaUCGCGCGguggugcagcucgAGCGCggucgGCCGCCGu -3' miRNA: 3'- aAGGGC--------------AGCGCGU-------------UCGCGa----CGGUGGC- -5' |
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18756 | 3' | -60.9 | NC_004683.1 | + | 53061 | 0.73 | 0.133642 |
Target: 5'- gUCuCCGUUGCGCcuGCGCUucuuucugGCCACCu -3' miRNA: 3'- aAG-GGCAGCGCGuuCGCGA--------CGGUGGc -5' |
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18756 | 3' | -60.9 | NC_004683.1 | + | 56071 | 0.7 | 0.220661 |
Target: 5'- --aCCGcCGCGUcuGGCGCUGgCACCGc -3' miRNA: 3'- aagGGCaGCGCGu-UCGCGACgGUGGC- -5' |
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18756 | 3' | -60.9 | NC_004683.1 | + | 56682 | 0.66 | 0.412049 |
Target: 5'- -aCCCGcggccCGCGguGGUGCUGCCuuUGg -3' miRNA: 3'- aaGGGCa----GCGCguUCGCGACGGugGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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