Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18758 | 5' | -54.6 | NC_004683.1 | + | 49109 | 0.72 | 0.404779 |
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Target: 5'- -cGGUAGCCGugCgaGCGCUucCCGGu -3' miRNA: 3'- cuCCAUUGGCugGaaCGCGGuuGGCU- -5' |
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| 18758 | 5' | -54.6 | NC_004683.1 | + | 49419 | 0.67 | 0.677983 |
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Target: 5'- cGAGGgcguucugaUGGCCGACCUgaaaacUGCCGACCu- -3' miRNA: 3'- -CUCC---------AUUGGCUGGAac----GCGGUUGGcu -5' |
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| 18758 | 5' | -54.6 | NC_004683.1 | + | 49429 | 0.66 | 0.742569 |
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Target: 5'- aGGGgcACCGGCCgucguggUGCgGgCAGCCGc -3' miRNA: 3'- cUCCauUGGCUGGa------ACG-CgGUUGGCu -5' |
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| 18758 | 5' | -54.6 | NC_004683.1 | + | 51305 | 0.66 | 0.76332 |
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Target: 5'- gGAGGgccGCCGAugaaCCUcaaCGCCAGCCGc -3' miRNA: 3'- -CUCCau-UGGCU----GGAac-GCGGUUGGCu -5' |
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| 18758 | 5' | -54.6 | NC_004683.1 | + | 53131 | 0.68 | 0.622841 |
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Target: 5'- gGAGGUGACCaugucGACgUUcGCGCCc-CCGAc -3' miRNA: 3'- -CUCCAUUGG-----CUGgAA-CGCGGuuGGCU- -5' |
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| 18758 | 5' | -54.6 | NC_004683.1 | + | 53616 | 0.73 | 0.360105 |
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Target: 5'- -uGGUGACCuugggcggauaGACCUugUGCGCgAGCCGGa -3' miRNA: 3'- cuCCAUUGG-----------CUGGA--ACGCGgUUGGCU- -5' |
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| 18758 | 5' | -54.6 | NC_004683.1 | + | 56881 | 0.66 | 0.753007 |
|
Target: 5'- -uGGUGAaggcccCCGACC-UGCGCCGAUg-- -3' miRNA: 3'- cuCCAUU------GGCUGGaACGCGGUUGgcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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