Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18759 | 5' | -59.8 | NC_004683.1 | + | 31798 | 0.66 | 0.498828 |
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Target: 5'- -aUGGUGGUGUCUCcgauguaGAACCGcccuaGGCCAa -3' miRNA: 3'- ccGCUACCGCAGAG-------CUUGGCc----CCGGU- -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 17312 | 0.66 | 0.506819 |
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Target: 5'- --aGAUGGC--CUCGAcguuguacgggggcGCCGGGGUCGg -3' miRNA: 3'- ccgCUACCGcaGAGCU--------------UGGCCCCGGU- -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 51356 | 0.66 | 0.509829 |
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Target: 5'- cGGCGAUGaGUGccucaccggccuUCUCccAGCCGaGGGCCu -3' miRNA: 3'- -CCGCUAC-CGC------------AGAGc-UUGGC-CCCGGu -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 18511 | 0.66 | 0.519917 |
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Target: 5'- aGCGGcaccGCGUUcaugUUGAACCGGGGCa- -3' miRNA: 3'- cCGCUac--CGCAG----AGCUUGGCCCCGgu -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 1480 | 0.66 | 0.519917 |
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Target: 5'- aGCGG-GGCGUcCUCGAucGCCGGcuugucguGCCAg -3' miRNA: 3'- cCGCUaCCGCA-GAGCU--UGGCCc-------CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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