Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18760 | 5' | -51.9 | NC_004683.1 | + | 53510 | 0.66 | 0.885181 |
Target: 5'- aGGuuGUCGUCCGcgucgaacgGGGUCcAAUCACCc-- -3' miRNA: 3'- -CCu-CGGCAGGU---------UCCAG-UUAGUGGaac -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 5489 | 0.66 | 0.869476 |
Target: 5'- gGGAuCCGUUgGAGGUgaUGAUCACCgUGg -3' miRNA: 3'- -CCUcGGCAGgUUCCA--GUUAGUGGaAC- -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 12475 | 0.68 | 0.79709 |
Target: 5'- -cGGCUGUCCGAGGcgcagCAGggCACCUg- -3' miRNA: 3'- ccUCGGCAGGUUCCa----GUUa-GUGGAac -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 2245 | 0.68 | 0.787123 |
Target: 5'- gGGAGCgGUUCGugAGGUUGAUCgACCg-- -3' miRNA: 3'- -CCUCGgCAGGU--UCCAGUUAG-UGGaac -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 3964 | 0.68 | 0.778008 |
Target: 5'- cGAGCCGaucaugggguggcggCCGuGGUCGAacugCACCUUGg -3' miRNA: 3'- cCUCGGCa--------------GGUuCCAGUUa---GUGGAAC- -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 43908 | 0.69 | 0.75626 |
Target: 5'- cGAGCgCGgCCGAGGUCAGcagcgCACCg-- -3' miRNA: 3'- cCUCG-GCaGGUUCCAGUUa----GUGGaac -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 17293 | 0.7 | 0.702346 |
Target: 5'- cGGGGgCG-CCGGGGUCGG-CACCaUGa -3' miRNA: 3'- -CCUCgGCaGGUUCCAGUUaGUGGaAC- -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 4263 | 0.7 | 0.680197 |
Target: 5'- cGAuGCgGUCCAAGGUgAuUgACCUUGg -3' miRNA: 3'- cCU-CGgCAGGUUCCAgUuAgUGGAAC- -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 44396 | 0.73 | 0.524581 |
Target: 5'- cGAGCCG-CaCGAGGUCGAUaCGCCa-- -3' miRNA: 3'- cCUCGGCaG-GUUCCAGUUA-GUGGaac -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 40671 | 0.78 | 0.285529 |
Target: 5'- -cGGCCuggCCGAGGUCGGUCACCUg- -3' miRNA: 3'- ccUCGGca-GGUUCCAGUUAGUGGAac -5' |
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18760 | 5' | -51.9 | NC_004683.1 | + | 4355 | 1.12 | 0.001512 |
Target: 5'- uGGAGCCGUCCAAGGUCAAUCACCUUGg -3' miRNA: 3'- -CCUCGGCAGGUUCCAGUUAGUGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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