Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18761 | 5' | -56.7 | NC_004683.1 | + | 53182 | 0.66 | 0.685848 |
Target: 5'- aCGAACGcCcCGGCcGaUGGCUcGGGGCGu -3' miRNA: 3'- -GCUUGCaGaGCCGcC-ACUGA-CCUCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 48253 | 0.66 | 0.675193 |
Target: 5'- uGAGCGaCgccgcCGGCGaGUGGCUGcGcAGCGa -3' miRNA: 3'- gCUUGCaGa----GCCGC-CACUGAC-C-UCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 41391 | 0.66 | 0.653776 |
Target: 5'- aCGAGuaccCGUCggcUGGCGGcgUGGCUGGuGUGg -3' miRNA: 3'- -GCUU----GCAGa--GCCGCC--ACUGACCuCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 37284 | 0.67 | 0.621532 |
Target: 5'- cCGGAuUGUCUUGGUGGguGC-GGAGCGa -3' miRNA: 3'- -GCUU-GCAGAGCCGCCacUGaCCUCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 56088 | 0.68 | 0.536539 |
Target: 5'- aCGAA-GUCggCGGCGGUGG-UGGuGGCGa -3' miRNA: 3'- -GCUUgCAGa-GCCGCCACUgACC-UCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 40312 | 0.69 | 0.515852 |
Target: 5'- aGGGCaUCgccgCGGCGGUGACccgauGAGCGa -3' miRNA: 3'- gCUUGcAGa---GCCGCCACUGac---CUCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 42576 | 0.73 | 0.297711 |
Target: 5'- cCGAGCG-CgccgCGGCGGUcggggccggucguccGGCUGGGGUGg -3' miRNA: 3'- -GCUUGCaGa---GCCGCCA---------------CUGACCUCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 21566 | 0.73 | 0.29482 |
Target: 5'- uGGGCGg--CGGCGGUGGCgGGcGCGg -3' miRNA: 3'- gCUUGCagaGCCGCCACUGaCCuCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 414 | 0.75 | 0.23548 |
Target: 5'- gCGGGCGgugauggCgcaGGCGGUGGCUGGcGCGg -3' miRNA: 3'- -GCUUGCa------Gag-CCGCCACUGACCuCGC- -5' |
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18761 | 5' | -56.7 | NC_004683.1 | + | 5859 | 1.09 | 0.000872 |
Target: 5'- gCGAACGUCUCGGCGGUGACUGGAGCGa -3' miRNA: 3'- -GCUUGCAGAGCCGCCACUGACCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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