Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18765 | 5' | -58.4 | NC_004683.1 | + | 13816 | 0.66 | 0.545089 |
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Target: 5'- cCCGgcGGUGUuuGGCAGc-GCAGGc -3' miRNA: 3'- aGGCaaCCGCAggCUGUCucCGUCCa -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 20539 | 0.66 | 0.534642 |
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Target: 5'- aCCGgcGGCa-CCGGCGGcaacGGCGGGUu -3' miRNA: 3'- aGGCaaCCGcaGGCUGUCu---CCGUCCA- -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 41262 | 0.66 | 0.534642 |
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Target: 5'- gUCGgcgGGUGUCCauaucgGACGGAcauuGGCGGGUa -3' miRNA: 3'- aGGCaa-CCGCAGG------CUGUCU----CCGUCCA- -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 28031 | 0.66 | 0.534642 |
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Target: 5'- ---aUUGGCGccgcCCGGC-GAGGCAGGg -3' miRNA: 3'- aggcAACCGCa---GGCUGuCUCCGUCCa -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 53840 | 0.66 | 0.524267 |
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Target: 5'- gCCGUUGGCGagCCGuGCAGuccagcugaucGGGCcGGUc -3' miRNA: 3'- aGGCAACCGCa-GGC-UGUC-----------UCCGuCCA- -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 23097 | 0.67 | 0.483599 |
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Target: 5'- cUCGUgGGCGUUCGcCAGcGGCAGcGUg -3' miRNA: 3'- aGGCAaCCGCAGGCuGUCuCCGUC-CA- -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 20721 | 0.67 | 0.473667 |
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Target: 5'- aUCCGUUGGCuGgagugCCGGguGGGcCAGGg -3' miRNA: 3'- -AGGCAACCG-Ca----GGCUguCUCcGUCCa -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 6357 | 0.68 | 0.454118 |
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Target: 5'- uUCCGUUGGgGUcagccaCCGAgGGGGcGUGGGg -3' miRNA: 3'- -AGGCAACCgCA------GGCUgUCUC-CGUCCa -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 55365 | 0.71 | 0.293376 |
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Target: 5'- gUCGggGuuGUUCGACAGcAGGCAGGUg -3' miRNA: 3'- aGGCaaCcgCAGGCUGUC-UCCGUCCA- -5' |
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| 18765 | 5' | -58.4 | NC_004683.1 | + | 9001 | 1.07 | 0.000746 |
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Target: 5'- uUCCGUUGGCGUCCGACAGAGGCAGGUu -3' miRNA: 3'- -AGGCAACCGCAGGCUGUCUCCGUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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