Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18766 | 3' | -55.4 | NC_004683.1 | + | 41286 | 0.71 | 0.440729 |
Target: 5'- gGCGUccUCggcgGCcuucgCCGCGUCGGCGggUGu -3' miRNA: 3'- gCGUA--AGaa--CG-----GGCGCAGCCGCuuGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 49063 | 0.67 | 0.672094 |
Target: 5'- uCGCGgcgg-GCCCGCGguacuuucCGGCGAuguuGCGg -3' miRNA: 3'- -GCGUaagaaCGGGCGCa-------GCCGCU----UGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 52609 | 0.67 | 0.693666 |
Target: 5'- gGCAUUcCUUGgCCGCcucGUCGGCc-GCGa -3' miRNA: 3'- gCGUAA-GAACgGGCG---CAGCCGcuUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 52726 | 0.67 | 0.672094 |
Target: 5'- uGCGUUCggcGCguuCCuCGUCGGUGAACa -3' miRNA: 3'- gCGUAAGaa-CG---GGcGCAGCCGCUUGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 56318 | 0.72 | 0.367776 |
Target: 5'- gCGCGUUCUcgaugucggguUGCCaGCGUCGGgGAuacugGCGg -3' miRNA: 3'- -GCGUAAGA-----------ACGGgCGCAGCCgCU-----UGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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