Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18769 | 3' | -53.2 | NC_004683.1 | + | 46327 | 0.66 | 0.775373 |
Target: 5'- gAACACCACGuacauGCGGUcccgUGaCUGCGGc- -3' miRNA: 3'- -UUGUGGUGCu----CGUUAa---AC-GACGCCca -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 48257 | 0.66 | 0.754305 |
Target: 5'- cGACGCCGCcGGCGAgugGCUGCGc-- -3' miRNA: 3'- -UUGUGGUGcUCGUUaaaCGACGCcca -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 43637 | 0.66 | 0.754305 |
Target: 5'- aGGCACC-CGAGaCGAUcucgcaagUGCUGCaGGUg -3' miRNA: 3'- -UUGUGGuGCUC-GUUAa-------ACGACGcCCA- -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 2161 | 0.67 | 0.710671 |
Target: 5'- --gGCCugGGGUggUcccugaUUGCcGCGGGUg -3' miRNA: 3'- uugUGGugCUCGuuA------AACGaCGCCCA- -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 49153 | 0.68 | 0.677033 |
Target: 5'- uGCugCGCGAGCGAcUggggagagcUGCUcugggGCGGGUa -3' miRNA: 3'- uUGugGUGCUCGUUaA---------ACGA-----CGCCCA- -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 14317 | 0.69 | 0.608838 |
Target: 5'- cGGCACagcuuGCG-GCAG--UGCUGCGGGUc -3' miRNA: 3'- -UUGUGg----UGCuCGUUaaACGACGCCCA- -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 47402 | 0.69 | 0.586163 |
Target: 5'- gAACACCACG-GCGAccUGCUcgaccaucGCGGGc -3' miRNA: 3'- -UUGUGGUGCuCGUUaaACGA--------CGCCCa -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 25347 | 0.7 | 0.552483 |
Target: 5'- cAGCGCgGCcAGCAGUgcgaccaGCUGCGGGa -3' miRNA: 3'- -UUGUGgUGcUCGUUAaa-----CGACGCCCa -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 26602 | 0.7 | 0.530352 |
Target: 5'- gAACACCGCccgguAGCAcuccaGCUGCGGGUc -3' miRNA: 3'- -UUGUGGUGc----UCGUuaaa-CGACGCCCA- -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 31289 | 0.71 | 0.508552 |
Target: 5'- uGCACCGgGGGUuGUgcaGCUGCGGGc -3' miRNA: 3'- uUGUGGUgCUCGuUAaa-CGACGCCCa -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 10475 | 0.71 | 0.48714 |
Target: 5'- gGACGCCuACGAcaaggcgcGCGA--UGCUGCGGGc -3' miRNA: 3'- -UUGUGG-UGCU--------CGUUaaACGACGCCCa -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 15614 | 0.72 | 0.415888 |
Target: 5'- cGugGCCGCGGGCAcgccGCUGCGGa- -3' miRNA: 3'- -UugUGGUGCUCGUuaaaCGACGCCca -5' |
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18769 | 3' | -53.2 | NC_004683.1 | + | 11230 | 1.05 | 0.002536 |
Target: 5'- gAACACCACGAGCAAUUUGCUGCGGGUc -3' miRNA: 3'- -UUGUGGUGCUCGUUAAACGACGCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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