Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18769 | 5' | -58.5 | NC_004683.1 | + | 36929 | 0.68 | 0.470501 |
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Target: 5'- uCGCCCCGGcgGUauCCcGGAggucagcuUGCGCu -3' miRNA: 3'- cGUGGGGCCuaCAgcGGaCCU--------ACGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 39612 | 0.67 | 0.490275 |
|
Target: 5'- gGCGCCCguuccgGGGUGggCGCCgccgGGGUG-GCg -3' miRNA: 3'- -CGUGGGg-----CCUACa-GCGGa---CCUACgCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 39701 | 0.69 | 0.369866 |
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Target: 5'- cCACCCCGGcgG-CGCCcaccccGGAacggGCGCc -3' miRNA: 3'- cGUGGGGCCuaCaGCGGa-----CCUa---CGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 46011 | 0.68 | 0.431293 |
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Target: 5'- uGCACUCCGGGc-UCGCCcagagaaUGucGGUGCGCg -3' miRNA: 3'- -CGUGGGGCCUacAGCGG-------AC--CUACGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 46064 | 0.68 | 0.451141 |
|
Target: 5'- cGUGCCCCGGu--UCGCCgag--GCGCa -3' miRNA: 3'- -CGUGGGGCCuacAGCGGaccuaCGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 49313 | 0.74 | 0.194989 |
|
Target: 5'- gGCGCCCCGGAcgugugGUUGCUggccgaucgGGAUaggGCGCg -3' miRNA: 3'- -CGUGGGGCCUa-----CAGCGGa--------CCUA---CGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 52064 | 0.67 | 0.510426 |
|
Target: 5'- cGCGCCCUGGcUGaUCGUCgacgcgGGAgGCGa -3' miRNA: 3'- -CGUGGGGCCuAC-AGCGGa-----CCUaCGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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