Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18774 | 3' | -59.2 | NC_004683.1 | + | 22824 | 0.7 | 0.33069 |
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Target: 5'- aCGAUU-CCAgGCGGCaCCGUAUCGAacGGUa -3' miRNA: 3'- -GCUGGuGGU-CGCCG-GGCAUAGCU--CCA- -5' |
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| 18774 | 3' | -59.2 | NC_004683.1 | + | 17220 | 0.71 | 0.272767 |
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Target: 5'- cCGACC-CCGGCGccCCCGUacaacGUCGAGGc -3' miRNA: 3'- -GCUGGuGGUCGCc-GGGCA-----UAGCUCCa -5' |
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| 18774 | 3' | -59.2 | NC_004683.1 | + | 20533 | 0.71 | 0.272767 |
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Target: 5'- aCGAUCACCGGCGGCaCCGg--CGgcaacggcGGGUu -3' miRNA: 3'- -GCUGGUGGUCGCCG-GGCauaGC--------UCCA- -5' |
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| 18774 | 3' | -59.2 | NC_004683.1 | + | 4943 | 0.72 | 0.223274 |
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Target: 5'- gCGGCgGCCAGCGGCgcaugCGcGUCGAGGc -3' miRNA: 3'- -GCUGgUGGUCGCCGg----GCaUAGCUCCa -5' |
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| 18774 | 3' | -59.2 | NC_004683.1 | + | 17882 | 0.73 | 0.191345 |
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Target: 5'- cCGugCGCCAGCGGCgucaCGUugaccucuUCGAGGa -3' miRNA: 3'- -GCugGUGGUCGCCGg---GCAu-------AGCUCCa -5' |
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| 18774 | 3' | -59.2 | NC_004683.1 | + | 14397 | 1.07 | 0.000709 |
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Target: 5'- cCGACCACCAGCGGCCCGUAUCGAGGUc -3' miRNA: 3'- -GCUGGUGGUCGCCGGGCAUAGCUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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